MPL W515L/K Mutations in Chronic Myeloproliferative Neoplasms
| Autor: | Veysel Sabri Hancer, Reyhan Diz-Kucukkaya, Timur Selcuk Akpinar, Meliha Nalcaci |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Pathology lcsh:Internal medicine Essential thrombocythemia medicine.disease_cause Gastroenterology law.invention Myeloproliferative neoplasms law Internal medicine hemic and lymphatic diseases medicine JAK-2 V617F mutation In patient Clinical significance Myelofibrosis lcsh:RC31-1245 Polymerase chain reaction Thrombopoietin receptor Mutation business.industry lcsh:RC633-647.5 Hematology lcsh:Diseases of the blood and blood-forming organs medicine.disease Peripheral blood Primary myelofibrosis business Research Article MPL W515L/K mutations |
| Zdroj: | Turkish Journal of Hematology Turkish Journal of Hematology, Vol 30, Iss 1, Pp 8-12 (2013) |
| ISSN: | 1308-5263 1300-7777 |
| Popis: | Objective: The MPL gene encodes the thrombopoietin receptor. Recently MPL mutations (MPL W515L or MPL W515K) were described in patients with essential thrombocythemia (ET) and primary (idiopathic) myelofibrosis (PMF). The prevalence and the clinical importance of these mutations are not clear. In the present study, we aimed to investigate the frequency and clinical significance of MPL W515L/K mutations in our patients with ET and PMF. Materials and Methods: A total of 77 patients (66 were diagnosed with ET and 11 with PMF) and 42 healthy controls were included in the study. Using peripheral blood samples, the presence of MPL W515L/K mutations and JAK-2 V617F mutation were analyzed by real-time polymerase chain reaction. Results: In our study, MPL W515L/K or JAK-2 V617F mutations were not observed in healthy controls. JAK-2 V617F mutation was present in 35 patients, of whom 29 had ET (43.9%, 29/66) and 6 had PMF (54.5%, 6/11). In the patient group, MPL W515L/K mutations were found in only 2 PMF cases, and these cases were negative for JAK-2 V617F mutation. The prevalence of MPL W515L/K mutations in the patient group was 2.6%, and the prevalence of MPL W515L/K mutations among the cases negative for the JAK-2 V617F mutation was found to be 4.8%. The 2 cases with MPL W515L/K mutations had long follow-up times (124 months and 71 months, respectively), had no thrombotic or hemorrhagic complications, and had no additional cytogenetic anomalies. Conclusion: MPL W515L/K mutations may be helpful for identifying clonal disease in MPN patients with no established Ph chromosome or JAK-2 V617F mutation. Conflict of interest:None declared. |
| Databáze: | OpenAIRE |
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