Interferon-beta Induces Selective Enhancement of Antigen-Specific T Cell Responses
| Autor: | David M. Essayan, Sarah E. Pacocha, Alfonso Oriente, Lawrence M. Lichtenstein, Shau Ku Huang |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cell division
medicine.medical_treatment T cell Immunology Epitopes T-Lymphocyte Biology Lymphocyte Activation Peripheral blood mononuclear cell Adjuvants Immunologic Downregulation and upregulation Antigen Virology medicine Humans Monocyte Toxoid Interferon-beta Cell Biology Molecular biology Clone Cells medicine.anatomical_structure Cytokine Leukocytes Mononuclear Mitogens Cell Division |
| Zdroj: | Journal of Interferon & Cytokine Research. 20:383-389 |
| ISSN: | 1557-7465 1079-9907 |
| Popis: | Interferon-beta (IFN-beta) inhibits mitogen-induced T cell responses, in part through downregulation of interleukin-12 (IL-12) or upregulation of IL-10. We have reexamined these findings using ragweed (RW) stimulated or tetanus toxoid (TT)-stimulated human peripheral blood mononuclear cells (PBMC) and nontransformed, antigen-specific, human Th0, Th1, and Th2 clones. IFN-beta induced concentration-dependent inhibition of phytohemagglutinin (PHA)-stimulated PBMC proliferation and enhancement of RW-stimulated or TTstimulated PBMC proliferation. Monocyte depletion of PBMC isolates resulted in concentration-dependent inhibition of RW-driven or TT-driven proliferation by IFN-beta. This response was unaltered by the addition of either exogenous recombinant human IL-12 (rHuIL-12) or saturating concentrations of anti-IL-10. Moreover, addition of exogenous rHuIL-10 to nondepleted RW-driven or TT-driven PBMC cultures did not alter the concentration-dependent enhancement of antigen-driven proliferation induced by IFN-beta. Th0, Th1, and Th2 clones stimulated in the presence of antigen and autologous, irradiated PBMC displayed concentration-dependent inhibition of proliferation in the presence of IFN-beta that was unaltered by the addition of either exogenous rHuIL-12 or a saturating concentration of anti-IL-10. Finally, whereas IFN-beta inhibited antigen-driven generation of IL-5, IL-12, IL-13, and IFN-gamma, IFN-beta enhanced generation of both IL-4 and IL-10. Thus, IFN-beta, induces a selective, IL-10-independent and IL-12-independent upregulation of antigen-specific T cell responses, supporting the role of IFN-beta as an immunomodulatory rather than an antiproliferative/immunosuppressive cytokine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|