Efficacy of recombinant Marek’s disease virus vectored vaccines with computationally optimized broadly reactive antigen (COBRA) hemagglutinin insert against genetically diverse H5 high pathogenicity avian influenza viruses
| Autor: | Teshome Mebatsion, Kateri Bertran, Ivette A. Nuñez, Dong-Hun Lee, Ted M. Ross, Aemro Kassa, Nikki Pritchard, Lindsay Killmaster, David E. Swayne, Miria Ferreira Criado, Mariana Sá e Silva |
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| Přispěvatelé: | Producció Animal, Sanitat Animal |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
030231 tropical medicine
Heterologous Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Biology medicine.disease_cause Antibodies Viral Virus Antigenic drift 03 medical and health sciences 0302 clinical medicine Antigen medicine Marek Disease Animals 030212 general & internal medicine Viral shedding Marek's disease Vaccines Synthetic General Veterinary General Immunology and Microbiology Influenza A Virus H5N1 Subtype Virulence Public Health Environmental and Occupational Health biology.organism_classification Virology Influenza A virus subtype H5N1 Infectious Diseases Hemagglutinins Influenza Vaccines Influenza in Birds biology.protein Molecular Medicine Chickens |
| Zdroj: | IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| Popis: | The genetic and antigenic drift associated with the high pathogenicity avian influenza (HPAI) viruses of Goose/Guangdong (Gs/GD) lineage and the emergence of vaccine-resistant field viruses underscores the need for a broadly protective H5 influenza A vaccine. Here, we tested experimental vector herpesvirus of turkey (vHVT)-H5 vaccines containing either wild-type clade 2.3.4.4A-derived H5 inserts or computationally optimized broadly reactive antigen (COBRA) inserts with challenge by homologous and genetically divergent H5 HPAI Gs/GD lineage viruses in chickens. Direct assessment of protection was confirmed for all the tested constructs, which provided clinical protection against the homologous and heterologous H5 HPAI Gs/GD challenge viruses and significantly decreased oropharyngeal shedding titers compared to the sham vaccine. The cross reactivity was assessed by hemagglutinin inhibition (HI) and focus reduction assay against a panel of phylogenetically and antigenically diverse H5 strains. The COBRA-derived H5 inserts elicited antibody responses against antigenically diverse strains, while the wild-type-derived H5 vaccines elicited protection mostly against close antigenically related clades 2.3.4.4A and 2.3.4.4D viruses. In conclusion, the HVT vector, a widely used replicating vaccine platform in poultry, with H5 insert provides clinical protection and significant reduction of viral shedding against homologous and heterologous challenge. In addition, the COBRA-derived inserts have the potential to be used against antigenically distinct co-circulating viruses and future drift variants. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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