Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line
| Autor: | László Pecze, Licun Wu, Marc de Perrot, Emanuela Felley-Bosco, Bart Vrugt, Beat Schwaller, Janine Worthmüller-Rodriguez, Walter Blum |
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| Přispěvatelé: | University of Zurich, Schwaller, Beat |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Mesothelioma
Lung Neoplasms Tumor suppressor gene Antigens Polyomavirus Transforming 610 Medicine & health Biology GPI-Linked Proteins 1309 Developmental Biology 1307 Cell Biology 03 medical and health sciences 0302 clinical medicine Cell Line Tumor 10049 Institute of Pathology and Molecular Pathology medicine Animals 030304 developmental biology Cell Line Transformed Cell Proliferation 0303 health sciences Neurofibromin 2 Cell growth Mesothelioma Malignant Epithelial Cells Cell Biology General Medicine medicine.disease Virology Xenograft Model Antitumor Assays Mice Mutant Strains 3. Good health Merlin (protein) Mice Inbred C57BL Cell culture 030220 oncology & carcinogenesis Mesothelin 10032 Clinic for Oncology and Hematology Cancer research Stem cell Erratum Fetal bovine serum Mesothelial Cell Developmental Biology |
| Zdroj: | In Vitro Cell.Dev.Biol.—Animal In vitro cellular & developmental biology. Animal |
| Popis: | Mesothelial cells are susceptible to 10:38 05.06.2015asbestos fiber-induced cytotoxicity and on longer time scales to transformation; the resulting mesothelioma is a highly aggressive neoplasm that is considered as incurable at the present time Zucali et al. (Cancer Treatment Reviews 37:543–558, 2011). Only few murine cell culture models of immortalized mesothelial cells and mesothelioma cell lines exist to date. We generated SV40-immortalized cell lines derived from wild-type (WT) and neurofibromatosis 2 (merlin) heterozygote (Nf2+/−) mice, both on a commonly used genetic background, C57Bl/6J. All immortalized mesothelial clones consistently grow in DMEM supplemented with fetal bovine serum. Cells can be passaged for more than 40 times without any signs of morphological changes or a decrease in proliferation rate. The tumor suppressor gene NF2 is one of the most frequently mutated genes in human mesothelioma, but its detailed function is still unknown. Thus, these genotypically distinct cell lines likely relevant for malignant mesothelioma formation are expected to serve as useful in vitro models, in particular to compare with in vivo studies in mice of the same genotype. Furthermore, we generated a novel murine mesothelioma cell line RN5 originating from an Nf2+/− mouse subjected to repeated crocidolite exposure. RN5 cells are highly tumorigenic. |
| Databáze: | OpenAIRE |
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