Fermentative metabolism of pyruvate by Rhodospirillum rubrum after anaerobic growth in darkness
| Autor: | R L Uffen, T E Gorrell |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Pyruvate decarboxylation
Formates Sodium chemistry.chemical_element Pyruvate Dehydrogenase Complex Biology Rhodospirillum rubrum Microbiology chemistry.chemical_compound Sodium pyruvate Formate Anaerobiosis Pyruvates Molecular Biology Cell-Free System Carbon Dioxide Darkness Hydrogen-Ion Concentration Pyruvate dehydrogenase complex biology.organism_classification Aldehyde Oxidoreductases Pyruvate carboxylase Bicarbonates chemistry Biochemistry Fermentation Hydrogen Research Article |
| Zdroj: | Journal of Bacteriology. 131:533-543 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.131.2.533-543.1977 |
| Popis: | Rhodospirillum rubrum grew anaerobically in darkness and fermented sodium pyruvate by a pyruvate formate-lyase reaction. During 30 min of anaerobic dark or light incubation with sodium pyrivate, crude extracts from fermentatively grown cells produced about 6 micronmol of acetylphosphate and formate per mg of protein in reactions performed at pH 8.3. Cell extracts also catalyzed the exchange of sodium [14C]formate into sodium pyruvate at an apparent pH optimum of 7.3 to 7.5, but only about 2.5 micronmol of acetylphosphate was produced at this lower pH value. R. rubrum may also form pyruvate:ferredoxin oxidoreductase activity, as evidenced by low bicarbonate exchange activity. However, its participation in pyruvate metabolism in anaerobic dark-grown cells was not understood. During anaerobic, dark growth with pyruvate, formate was an intermediate in H2 and CO2 gas evolution. In contrast with H2 production by a light-dependent H2-nitrogenase system in photosynthetically grown cells, H2 formation in fermenting R. rubrum occurred through a carbon monoxide-sensitive formic hydrogenlyase reaction not influenced by light. |
| Databáze: | OpenAIRE |
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