Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term
| Autor: | Ina Stuen, Lene Østby, Arne Reimers, Eirik Sundby |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
UGT1A4 Glucuronosyltransferase Clinical chemistry Placenta Blotting Western Biology Lamotrigine Isozyme Gene Expression Regulation Enzymologic Western blot Pregnancy Microsomes Internal medicine medicine Humans Pharmacology (medical) reproductive and urinary physiology Pharmacology Original Paper Epilepsy medicine.diagnostic_test Triazines medicine.disease Pregnancy Complications Endocrinology medicine.anatomical_structure Case-Control Studies embryonic structures Microsome biology.protein Anticonvulsants Female UGT |
| Zdroj: | European Journal of Drug Metabolism and Pharmacokinetics |
| ISSN: | 2107-0180 0378-7966 |
| DOI: | 10.1007/s13318-010-0021-x |
| Popis: | The placenta contains a large variety of metabolizing enzymes, among them UDP-glucuronosyltransferase (UGT). Several UGT2B isozymes have so far been detected in human placenta, but little is known on placental expression of UGT1A isozymes. The antiepileptic drug lamotrigine (LTG) is a UGT1A4-substrate, and its serum concentration falls by over 50% during pregnancy, leading to impaired seizure control. The placenta may be involved in this. Microsomes from term placentas of 4 LTG-users and 10 healthy control subjects were prepared. Western blot analysis detected UGT1A proteins in all placentas. The presence of UGT1A4 in placenta from LTG users was confirmed with UGT1A4 commercial standard and a specific UGT1A4 primary antibody. Since LTG is primarily metabolized by UGT1A4 and this isozyme is shown to be present in placenta at term, it may be hypothesized that the placenta is involved in the fall of LTG serum concentrations during pregnancy. |
| Databáze: | OpenAIRE |
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