IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly
| Autor: | Javier García-Nafría, Ingo H. Greger, Jake F. Watson |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Recombination Genetic Multidisciplinary 030102 biochemistry & molecular biology Cloning (programming) Bacteria Mutagenesis (molecular biology technique) Molecular cloning Biology In Vitro Techniques Article Single tube 03 medical and health sciences Transformation (genetics) 030104 developmental biology In vivo Mutagenesis Site-Directed Cloning Molecular Homologous recombination Cloning procedures |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In vivo homologous recombination holds the potential for optimal molecular cloning, however, current strategies require specialised bacterial strains or laborious protocols. Here, we exploit a recA-independent recombination pathway, present in widespread laboratory E.coli strains, to develop IVA (InVivoAssembly) cloning. This system eliminates the need for enzymatic assembly and reduces all molecular cloning procedures to a single-tube, single-step PCR, performed in |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|