Polymer Nanofiber-Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells
| Autor: | Wei-Han OuYang, Qinglin Shen, Roger S. Lo, Dongxia Wu, Juehua Yu, Xingzhong Zhao, Libo Zhao, Shuang Hou, Xiaochun Xu, Antoni Ribas, Charles Ng, Hsian-Rong Tseng, Mitch A. Garcia, Xiaohong Shi, Thomas H. Lee, Rongxian He, Xiangju Kong, Min Song, F. Charles Brunicardi |
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| Rok vydání: | 2013 |
| Předmět: |
Proto-Oncogene Proteins B-raf
Erythrocytes Polymers Nanofibers Nanotechnology Cell Separation Immunomagnetic separation Catalysis Article Circulating tumor cell Single-cell analysis Polylactic Acid-Polyglycolic Acid Copolymer Cell Line Tumor Medicine Humans Lactic Acid Melanoma business.industry Cancer General Chemistry General Medicine Microfluidic Analytical Techniques medicine.disease Neoplastic Cells Circulating Molecular analysis Nanofiber Cancer cell Mutation Cancer research Single-Cell Analysis business Polyglycolic Acid |
| Zdroj: | Angewandte Chemie. 125:3463-3467 |
| ISSN: | 0044-8249 |
| DOI: | 10.1002/ange.201208452 |
| Popis: | Circulating tumor cells (CTCs)[1] are cancer cells shed from either the primary tumors or metastatic sites. The presence and number of CTCs in peripheral blood can provide clinically significant data on prognosis and therapeutic response patterns, respectively[2]. Thus, as with traditional invasive tumor biopsies that enable gold-standard pathological analysis, CTCs can be regarded as “liquid biopsies” of the tumor, which enable repeated and relatively non-invasive characterization of tumor evolution, especially important during therapeutic interventions. Currently, FDA-cleared CellSearch™ Assay is costly and inefficient in capturing CTCs, and the enriched CTCs are typically contaminated with a large number of white blood cells (WBCs). As a result, the diagnostic value of CTCs has been underutilized. Over the past decade, a diversity of CTC detection technologies[2d, 3] have been developed to overcome the challenges encountered by the immunomagnetic separation-based CellSearch™ Assay. |
| Databáze: | OpenAIRE |
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