Extra copy of the mitochondrial cytochrome-c peroxidase gene confers a pyruvate-underproducing characteristic of sake yeast through respiratory metabolism
| Autor: | Yuki Yamamoto, Nairui Zhang, Yuki Kusaba, Tetsuro Kakeshita, Huanghuang Dai, Yuki Fujimaru, Mitsuhiro Takasaki, Hiroshi Kitagaki |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Copy Number Variations Gene Dosage Bioengineering Applied Microbiology and Biotechnology Saccharomyces cerevisiae Mitochondrion 01 natural sciences Gene Expression Regulation Enzymologic 03 medical and health sciences Oxygen Consumption 010608 biotechnology Gene Expression Regulation Fungal Pyruvic Acid Gene chemistry.chemical_classification Reactive oxygen species biology Organisms Genetically Modified Chemistry Cytochrome c peroxidase Alcoholic Beverages Chromosome Cytochrome-c Peroxidase Aneuploidy Yeast 030104 developmental biology Biochemistry Fermentation biology.protein Energy Metabolism Reactive Oxygen Species Metabolic Networks and Pathways Biotechnology Peroxidase |
| Zdroj: | Journal of bioscience and bioengineering. 131(6) |
| ISSN: | 1347-4421 |
| Popis: | The mechanism of pyruvate-underproduction of aneuploid sake yeast was investigated in this study. In our previous report, we revealed that an increase in chromosome XI decreases pyruvate productivity of sake yeast. In this report, we found that increased copy number of CCP1, which is located on chromosome XI and encodes cytochrome-c peroxidase, decreased the pyruvate productivity of sake yeasts. Introducing an extra copy of CCP1 activated respiratory metabolism governed by Hap4 and increased reactive oxygen species. Therefore, it was concluded that increased copy number of CCP1 on chromosome XI activated respiratory metabolism and decreased pyruvate levels in an aneuploid sake yeast. This is the first report that describes a mechanism underlying the improvement of brewery yeast by chromosomal aneuploidy. |
| Databáze: | OpenAIRE |
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