Indolopyridones Inhibit Human Immunodeficiency Virus Reverse Transcriptase with a Novel Mechanism of Action
| Autor: | Piet Wigerinck, Maryam Ehteshami, Jerome Deval, Bertrand Van Schoubroeck, Tania Ivens, Marcia Van Ginderen, Eva Bettens, Dirk Jochmans, Matthias Götte, Inky De Baere, Pascale Dehertogh, Kurt Hertogs, Bart Rudolf Romanie Kesteleyn, Herwig Van Marck |
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| Rok vydání: | 2006 |
| Předmět: |
DNA Replication
Indoles Pyridones Immunology Electrophoretic Mobility Shift Assay Biology medicine.disease_cause Microbiology Virology Vaccines and Antiviral Agents Nitriles medicine Nucleotide Binding site Mode of action DNA Primers chemistry.chemical_classification Mutation Sequence Analysis DNA Nucleotidyltransferase Resistance mutation Molecular biology HIV Reverse Transcriptase Reverse transcriptase Kinetics chemistry Insect Science HIV-1 Reverse Transcriptase Inhibitors Primer (molecular biology) |
| Zdroj: | Journal of Virology. 80:12283-12292 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | We have discovered a novel class of human immunodeficiency virus (HIV) reverse transcriptase (RT) inhibitors that block the polymerization reaction in a mode distinct from those of the nucleoside or nucleotide RT inhibitors (NRTIs) and nonnucleoside RT inhibitors (NNRTIs). For this class of indolopyridone compounds, steady-state kinetics revealed competitive inhibition with respect to the nucleotide substrate. Despite substantial structural differences with classical chain terminators or natural nucleotides, these data suggest that the nucleotide binding site of HIV RT may accommodate this novel class of RT inhibitors. To test this hypothesis, we have studied the mechanism of action of the prototype compound indolopyridone-1 (INDOPY-1) using a variety of complementary biochemical tools. Time course experiments with heteropolymeric templates showed “hot spots” for inhibition following the incorporation of pyrimidines (T>C). Moreover, binding studies and site-specific footprinting experiments revealed that INDOPY-1 traps the complex in the posttranslocational state, preventing binding and incorporation of the next complementary nucleotide. The novel mode of action translates into a unique resistance profile. While INDOPY-1 susceptibility is unaffected by mutations associated with NNRTI or multidrug NRTI resistance, mutations M184V and Y115F are associated with decreased susceptibility, and mutation K65R confers hypersusceptibility to INDOPY-1. This resistance profile provides additional evidence for active site binding. In conclusion, this class of indolopyridones can occupy the nucleotide binding site of HIV RT by forming a stable ternary complex whose stability is mainly dependent on the nature of the primer 3′ end. |
| Databáze: | OpenAIRE |
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