Monitoring neutropenia for cancer patients at the point of care
Autor: | Naside Gozde Durmus, Utkan Demirci, Thomas J. Metzner, Yoriko Imae, Christian R. Hoerner, Jordan S. Preiss, Heather A. Wakelee, Mehmet Ozsoz, Huseyin Cumhur Tekin, Alice C. Fan, James L. Kingsley, Hakan Inan, Erkan Tüzel, Mehmet Ozgun Ozen |
---|---|
Přispěvatelé: | Tekin, Hüseyin Cumhur, Izmir Institute of Technology. Biotechnology and Bioengineering |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Neutropenia medicine.medical_treatment Article 03 medical and health sciences 0302 clinical medicine Internal medicine medicine General Materials Science Whole blood Point of care Chemotherapy Cancer monitoring business.industry Neutrophil Cancer General Chemistry Phlebotomy medicine.disease Lens-free shadow imaging 030104 developmental biology Microfluidic 030220 oncology & carcinogenesis Immunology Cohort Poisson distribution business Kidney cancer |
Popis: | PubMed: 30740513 Neutrophils have a critical role in regulating the immune system. The immune system is compromised during chemotherapy, increasing infection risks and imposing a need for regular monitoring of neutrophil counts. Although commercial hematology analyzers are currently used in clinical practice for neutrophil counts, they are only available in clinics and hospitals, use large blood volumes, and are not available at the point of care (POC). Additionally, phlebotomy and blood processing require trained personnel, where patients are often admitted to hospitals when the infections are at late stage due to lack of frequent monitoring. Here, a reliable method is presented that selectively captures and quantifies white blood cells (WBCs) and neutrophils from a finger prick volume of whole blood by integrating microfluidics with high-resolution imaging algorithms. The platform is compact, portable, and easy to use. It captures and quantifies WBCs and neutrophils with high efficiency (> 95%) and specificity (> 95%) with an overall 4.2% bias compared to standard testing. The results from a small cohort of patients (N = 11 healthy, N = 5 lung and kidney cancer) present a unique disposable cell counter, demonstrating the ability of this tool to monitor neutrophil and WBC counts within clinical or in resource-constrained environments. |
Databáze: | OpenAIRE |
Externí odkaz: |