Comprehensive Transcriptome Reveals an Opposite Regulatory Effect of Plant Growth Retardants in Controlling Seedling Overgrowth between Roots and Shoots

Autor: Guanxing Chen, Wu Zhanhui, Qiwen Zhong, Mingchi Liu, Ji Yanhai, Xuyang Zheng, Mingyun Zhang, Changlong Wen
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
tomato
01 natural sciences
Plant Roots
Transcriptome
lcsh:Chemistry
chemistry.chemical_compound
Solanum lycopersicum
Plant Growth Regulators
Gene Expression Regulation
Plant
plant growth retardants
lcsh:QH301-705.5
Spectroscopy
biology
Phytochrome
food and beverages
General Medicine
Computer Science Applications
Organ Specificity
Shoot
Gibberellin
Chlormequat
Plant Shoots
Signal Transduction
gibberellins
Plant Development
Genes
Plant
Catalysis
Article
Paclobutrazol
Inorganic Chemistry
03 medical and health sciences
Botany
Physical and Theoretical Chemistry
Molecular Biology
Gene
overgrowth
Organic Chemistry
Triazoles
biology.organism_classification
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Seedling
Seedlings
transcriptome
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 13, p 3307 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 13
ISSN: 1422-0067
Popis: Seedling overgrowth always develops in undernourished plants due to biotic or abiotic stresses, which significantly decrease the yield of crops and vegetables. It is known that the plant growth retardants paclobutrazol (PBZ) and chlormequat chloride (CCC) are the most commonly used chemicals in controlling seedling height in plants by regulating the gibberellin (GA) biosynthesis pathway. However, the exact molecular regulation mechanism remains largely unknown. This study performed a comprehensive transcriptome profile to identify significantly differentially expressed genes after adding CCC and PBZ to the water culture seedling raising system for the first time. According to the obviously restrained shoots and roots, the GA biosynthesis genes were significantly decreased, as well as the endogenous GA content being reduced. Intriguingly, the GA signaling pathway genes were affected in opposite ways, increasing in roots but decreasing in shoots, especially regarding the phytochrome interacting factor SlPIF1 and the downstream genes expansins (SlEXPs), which promote cell wall remodeling. Further study found that the most down-regulated genes SlEXPA5 and SlEXPA15 were expressed specifically in shoot tissue, performing the function of repressing elongation, while the up-regulated genes SlEXPB2 and SlEXPB8 were proven to be root-specific expressed genes, which may promote horizontal elongation in roots. This research reported the comprehensive transcriptome profiling of plant growth retardants in controlling seedling overgrowth and restraining GA biosynthesis through the regulation of the GA signaling-related genes SlPIF1 and SlEXPs, with an opposite expression pattern between roots and shoots.
Databáze: OpenAIRE
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