An easy, inexpensive, and sensitive method for the quantification of chitin in insect peritrophic membrane by image processing
| Autor: | Naoki Mori, Naoko Yoshinaga, Masayoshi Teraishi, Yutaka Okumoto, Ryu Nakata |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecta media_common.quotation_subject Image processing Chitin Insect engineering.material 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Chitosan 03 medical and health sciences chemistry.chemical_compound Limit of Detection Animals Molecular Biology media_common Organic Chemistry General Medicine 010602 entomology 030104 developmental biology Membrane chemistry Larva engineering Biophysics Insect Proteins Biopolymer Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 83(9) |
| ISSN: | 1347-6947 |
| Popis: | Chitin, poly (β-(1→4)-N-acetyl-d-glucosamine), is an important biopolymer for insects that is utilized as a major component of peritrophic membrane. The chitin content in peritrophic membrane is of expedient interest from a pest control perspective, although it is hard to quantify chitin. In this study, we establish a facile method for the quantification of chitin in peritrophic membrane by image processing. In this method, chitin was indirectly quantified using chitosan–I3− complex, which exhibited a specific red-purple color. A calibration curve using a chitosan solution showed good linearity in a concentration range of 0.05–0.5 μg/μL. We quantified the amount of chitin in peritrophic membrane of Spodoptera litura (Lepidoptera: Noctuidae) larvae using this method. Throughout the study, only common inexpensive regents and easily attainable apparatuses were employed. This method can be easily applied to the sensitive quantification of the amounts of chitin and chitosan in materials by wide range of researchers.Abbreviations: LOD: limit of detection; LOQ: limit of quantification; ROI: region of interest; RSD: relative standard deviation. |
| Databáze: | OpenAIRE |
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