Novel flow cytometric approach for the detection of adipocyte subpopulations during adipogenesis[S]
| Autor: | Michael S. Pepper, Fiona A. van Vollenstee, Carla Dessels, Danielle de Villiers, Karlien Kallmeyer, Chrisna Durandt, Candice Murdoch, Marnie Potgieter |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CD36 Antigens Stromal cell lipid droplet QD415-436 Biology Nile Red Fatty Acid-Binding Proteins Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Downregulation and upregulation Lipid droplet Adipocyte Bodipy 493/503 Gene expression Adipocytes CCAAT-Enhancer-Binding Protein-alpha Methods fatty/acid binding protein Humans triglyceride adipocyte differentiation Adipogenesis Cluster of differentiation Mesenchymal stem cell adipose tissue-derived stromal cells Cell Biology Flow Cytometry Cell biology PPAR gamma 030104 developmental biology chemistry Gene Expression Regulation Microscopy Fluorescence 030220 oncology & carcinogenesis Immunology cluster of differentiation 36 gene expression |
| Zdroj: | Journal of Lipid Research, Vol 57, Iss 4, Pp 729-742 (2016) Journal of Lipid Research |
| ISSN: | 0022-2275 |
| Popis: | The ability of mesenchymal stromal cells (MSCs) to differentiate into adipocytes provides a cellular model of human origin to study adipogenesis in vitro. One of the major challenges in studying adipogenesis is the lack of tools to identify and monitor the differentiation of various subpopulations within the heterogeneous pool of MSCs. Cluster of differentiation (CD)36 plays an important role in the formation of intracellular lipid droplets, a key characteristic of adipocyte differentiation/maturation. The objective of this study was to develop a reproducible quantitative method to study adipocyte differentiation by comparing two lipophilic dyes [Nile Red (NR) and Bodipy 493/503] in combination with CD36 surface marker staining. We identified a subpopulation of adipose-derived stromal cells that express CD36 at intermediate/high levels and show that combining CD36 cell surface staining with neutral lipid-specific staining allows us to monitor differentiation of adipose-derived stromal cells that express CD36(intermediate/high)during adipocyte differentiation in vitro. The gradual increase of CD36(intermediate/high/)NR(positive)cells during the 21 day adipogenesis induction period correlated with upregulation of adipogenesis-associated gene expression. |
| Databáze: | OpenAIRE |
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