Transcutaneous Immunization with a Vibrio cholerae O1 Ogawa Synthetic Hexasaccharide Conjugate following Oral Whole-Cell Cholera Vaccination Boosts Vibriocidal Responses and Induces Protective Immunity in Mice
| Autor: | Mohammad Arifuzzaman, Daniel T. Leung, Jason B. Harris, Md. Saruar Bhuiyan, Firdausi Qadri, Anuj Kalsy, Richelle C. Charles, Edward T. Ryan, John D. Clements, Sean M. Rollins, P. Kovac, Rina Saksena, Stephen B. Calderwood, Regina C. LaRocque, Alaullah Sheikh, Abdullah A. Tarique |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Blood Bactericidal Activity Cholera Toxin Injections Subcutaneous medicine.medical_treatment Bacterial Toxins Clinical Biochemistry Immunology Immunization Secondary Administration Oral Oligosaccharides Biology Administration Cutaneous Vaccines Attenuated medicine.disease_cause Microbiology Enterotoxins Feces Mice Immune system Adjuvants Immunologic Cholera medicine Animals Immunology and Allergy Vaccines Antigens Bacterial Vaccines Conjugate Escherichia coli Proteins Feces analysis Cholera toxin Vibrio cholerae O1 Cholera Vaccines medicine.disease Antibodies Bacterial Survival Analysis Immunoglobulin A Vaccination Disease Models Animal Immunoglobulin M Vibrio cholerae Immunoglobulin G Female Cholera vaccine Adjuvant |
| Zdroj: | Clinical and Vaccine Immunology. 19:594-602 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | A shortcoming of currently available oral cholera vaccines is their induction of relatively short-term protection against cholera compared to that afforded by wild-type disease. We were interested in whether transcutaneous or subcutaneous boosting using a neoglycoconjugate vaccine made from a synthetic terminal hexasaccharide of the O-specific polysaccharide ofVibrio choleraeO1 (Ogawa) coupled to bovine serum albumin as a carrier (CHO-BSA) could boost lipopolysaccharide (LPS)-specific and vibriocidal antibody responses and result in protective immunity following oral priming immunization with whole-cell cholera vaccine. We found that boosting with CHO-BSA with immunoadjuvantative cholera toxin (CT) orEscherichia coliheat-labile toxin (LT) following oral priming with attenuatedV. choleraeO1 vaccine strain O395-NT resulted in significant increases in serum anti-V. choleraeLPS IgG, IgM, and IgA (P< 0.01) responses as well as in anti-Ogawa (P< 0.01) and anti-Inaba (P< 0.05) vibriocidal titers in mice. The LPS-specific IgA responses in stool were induced by transcutaneous (P< 0.01) but not subcutaneous immunization. Immune responses following use of CT or LT as an adjuvant were comparable. In a neonatal mouse challenge assay, immune serum from boosted mice was associated with 79% protective efficacy against death. Our results suggest that transcutaneous and subcutaneous boosting with a neoglycoconjugate following oral cholera vaccination may be an effective strategy to prolong protective immune responses againstV. cholerae. |
| Databáze: | OpenAIRE |
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