Relationship between Allelic Heterozygosity in BoLA-DRB3 and Proviral Loads in Bovine Leukemia Virus-Infected Cattle
| Autor: | Kosuke Notsu, Junzo Norimine, Satoshi Sekiguchi, Ngan Thi Mai, Shuya Mitoma, Chiho Kaneko, Eslam Elhanafy, Huyen Thi Nguyen, Hala El Daous |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
RFLP-PCR
BoLA-DRB3 allele combinations 040301 veterinary sciences animal diseases viruses proviral load Human leukocyte antigen Genome Article law.invention Restriction fragment 0403 veterinary science Loss of heterozygosity 03 medical and health sciences law lcsh:Zoology lcsh:QL1-991 Allele Polymerase chain reaction 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary Bovine leukemia virus biology heterozygous alleles virus diseases 04 agricultural and veterinary sciences biochemical phenomena metabolism and nutrition biology.organism_classification Virology Enzootic Bovine Leukosis bovine leukemia virus biology.protein lcsh:SF600-1100 Animal Science and Zoology |
| Zdroj: | Animals Volume 11 Issue 3 Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 647, p 647 (2021) |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani11030647 |
| Popis: | Enzootic bovine leukosis is a lethal neoplastic disease caused by bovine leukemia virus (BLV), belongs to family Retroviridae. The BLV proviral load (PVL) represents the quantity of BLV genome that has integrated into the host’s genome in BLV-infected cells. Bovine leukocyte antigen (BoLA) class II allelic polymorphisms are associated with PVLs in BLV-infected cattle. We sought to identify relationships between BoLA-DRB3 allelic heterozygosity and BLV PVLs among different cattle breeds. Blood samples from 598 BLV-infected cattle were quantified to determine their PVLs by real-time polymerase chain reaction. The results were confirmed by a BLV-enzyme-linked immunosorbent assay. Restriction fragment length polymorphism-polymerase chain reaction identified 22 BoLA-DRB3 alleles. Multivariate negative binomial regression modeling was used to test for associations between BLV PVLs and BoLA-DRB3 alleles. BoLA-DRB3.2*3, *7, *8, *11, *22, *24, and *28 alleles were significantly associated with low PVLs. BoLA-DRB3.2*10 was significantly associated with high PVLs. Some heterozygous allele combinations were associated with low PVLs (*3/*28, *7/*8, *8/*11, *10/*11, and *11/*16) others were associated with high PVLs (*1/*41, *10/*16, *10/*41, *16/*27, and *22/*27). Interestingly, the BoLA-DRB3.2*11 heterozygous allele was always strongly and independently associated with low PVLs. This is the first reported evidence of an association between heterozygous allelic combinations and BLV PVLs. |
| Databáze: | OpenAIRE |
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