Reduced synovial inflammation and inhibition of matrix metalloproteinases explicates anti-osteoarthritis activity of polyherbal formulations
| Autor: | Soumya J. Koppikar, Abhay Harsulkar, Dhanashri R Ingale, Priya Kulkarni, Alpana Moghe, Suresh Jagtap |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
malondialdehyde
0301 basic medicine Lipopolysaccharide Cell Survival Anti-Inflammatory Agents nitric-oxide Osteoarthritis Pharmacology Nitric Oxide medicine.disease_cause Antioxidants Cell Line Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Synovitis medicine Animals Gelatinase Pharmacology (medical) Collagenases Plant Extracts business.industry N-(3-[2-Furyl] acryloyl)-Leu-Gly-Pro-Ala medicine.disease Malondialdehyde Synoviocytes Matrix Metalloproteinases Medicine Ayurvedic osteoarthritis 030104 developmental biology chemistry 030220 oncology & carcinogenesis Collagenase Lipid Peroxidation Plant Preparations Rabbits Collagen business Oxidative stress Research Article medicine.drug |
| Zdroj: | Indian Journal of Pharmacology |
| ISSN: | 0253-7613 |
| DOI: | 10.4103/ijp.ijp_29_17 |
| Popis: | OBJECTIVES: Current osteoarthritis (OA) research experiences an incline toward Ayurveda to attain a complete cure without notable adverse effects. Ayurveda uses natural products, which are known to perform the multi-faceted role, a much demanding approach for OA management. However, lack of scientific evidence is a major drawback hindering their wider use. The present work investigated the anti-arthritic potential of Ashwagandharishta, Balarishta, Dashmoolarishta, and Triphala-extract to establish molecular-evidence for their clinical use. MATERIALS AND METHODS: Rabbit synoviocytes were induced using interleukin-1 beta (IL-1 β) and lipopolysaccharide (LPS) separately and were further treated with study formulations to test anti-inflammatory and anti-oxidant potential, using nitric oxide (NO) and malondialdehyde (MDA) assays. Collagenase inhibition activity was estimated with N-(3-[2-Furyl] acryloyl)-Leu-Gly-Pro-Ala (FALGPA)-substrate and gelatinase spot assays. Data were analyzed with GraphPad Prism using one-way ANOVA followed by Bonferroni's multiple comparison. RESULTS: The study formulations were effective against synovitis, oxidative-stress, and inhibiting collagenase. They caused NO reduction in selected concentrations. DA showed the maximum NO decline of 0.02 ± 0 and 0.97 ± 0.62 μM/ml with IL-1 β and LPS induction at 5 and 20 μg/ml concentrations, respectively. Estimated by FALGPA assay, increasing collagenase inhibition was observed as the function of concentration. All formulations showed a significant MDA decline, in dose-dependent manner. CONCLUSION: We assessed the anti-OA efficacy of conventionally prescribed Ayurvedic drugs using relevant biochemical assays. The studied formulations revealed potential to restrain synovitis, cartilage degeneration and to reduce oxidative stress, and the signature OA features. With established molecular authenticity, Ayurvedic drugs can offer a safer and affordable therapeutic option for OA. |
| Databáze: | OpenAIRE |
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