A Bioorthogonal Near-Infrared Fluorogenic Probe for mRNA Detection
| Autor: | Haoxing Wu, Neal K. Devaraj, Shuaijiang Jin, Seth C. Alexander |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cell Survival
Infrared Rays CHO Cells Conjugated system 010402 general chemistry Photochemistry 01 natural sciences Biochemistry Catalysis Antisense nucleic acid chemistry.chemical_compound Tetrazine Colloid and Surface Chemistry Cricetulus Animals Reactivity (chemistry) RNA Messenger Cyanine Fluorescent Dyes 010405 organic chemistry Chemistry RNA General Chemistry Combinatorial chemistry 0104 chemical sciences Nucleic acid Bioorthogonal chemistry |
| Zdroj: | Journal of the American Chemical Society. 138(36) |
| ISSN: | 1520-5126 |
| Popis: | There is significant interest in developing methods that visualize and detect RNA. Bioorthogonal template-driven tetrazine ligations could be a powerful route to visualizing nucleic acids in native cells, yet past work has been limited with respect to the diversity of fluorogens that can be activated via a tetrazine reaction. Herein we report a novel bioorthogonal tetrazine uncaging reaction that harnesses tetrazine reactivity to unmask vinyl ether caged fluorophores spanning the visible spectrum, including a near-infrared (NIR)-emitting cyanine dye. Vinyl ether caged fluorophores and tetrazine partners are conjugated to high-affinity antisense nucleic acid probes, which show highly selective fluorogenic reactivity when annealed to their respective target RNA sequences. A target sequence in the 3' untranslated region of an expressed mRNA was detected in live cells employing appropriate nucleic acid probes bearing a tetrazine-reactive NIR fluorogen. Given the expansion of tetrazine fluorogenic chemistry to NIR dyes, we believe highly selective proximity-induced fluorogenic tetrazine reactions could find broad uses in illuminating endogenous biomolecules in cells and tissues. |
| Databáze: | OpenAIRE |
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