Simple determination of urine cefazolin concentration in pediatric patients with urinary tract infections using high‐performance liquid chromatography

Autor:	Kurumi Abe, Kenji Momo, Yoshifusa Abe, Takeru Kanazawa, Ryo Karato, Katsumi Tanaka, Tadanori Sasaki
Rok vydání:	2022
Předmět:	Pharmacology Clinical Biochemistry Microbial Sensitivity Tests General Medicine Biochemistry Anti-Bacterial Agents Analytical Chemistry Cefazolin Urinary Tract Infections Drug Discovery Humans Child Molecular Biology Chromatography High Pressure Liquid
Zdroj:	Biomedical Chromatography. 36
ISSN:	1099-0801 0269-3879
DOI:	10.1002/bmc.5495
Popis:	Recently, global health concerns regarding increasing multidrug resistance have arisen. This study aimed to develop a simple, inexpensive and rapid high-performance liquid chromatography-ultraviolet (HPLC-UV) method for determining urinary concentrations of a first-generation cephem antibiotic in pediatric patients with urinary tract infections (UTIs). HPLC-UV was used to analyze urinary cefazolin concentrations at a detection wavelength of 254 nm. The assay used contained 10-fold diluted urine with an internal standard (cephapirin). The standard calibration curve for cefazolin was linear in the concentration range of 31.25-500 μg/ml (r
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aec48ab16dc441bc0b2a0c29f441c6c1 https://doi.org/10.1002/bmc.5495 Zobrazit plný text záznamu Plný text