Immunization with potato plants expressing VP60 protein protects against rabbit hemorrhagic disease virus
| Autor: | Francisco Parra, M. S. Marin, José M. Martín-Alonso, S. Castañón, José Antonio Boga, Ricardo J. Ordás, Jaime M. Humara, Rosa Casais |
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| Předmět: |
Hemorrhagic Disease Virus
Rabbit Transgene Recombinant Fusion Proteins Immunology Biology Microbiology Virus law.invention Capsid law Virology Animals Enhancer Antigens Viral Caliciviridae Infections Solanum tuberosum Viral Structural Proteins Vaccines Synthetic Antibody titer food and beverages Promoter Viral Vaccines biology.organism_classification Plants Genetically Modified Insect Science Recombinant DNA Pathogenesis and Immunity Cauliflower mosaic virus Rabbits |
| Zdroj: | Scopus-Elsevier ResearcherID |
| Popis: | The major structural protein VP60 of rabbit hemorrhagic disease virus (RHDV) has been produced in transgenic potato plants under the control of a cauliflower mosaic virus 35S promoter or a modified 35S promoter that included two copies of a strong transcriptional enhancer. Both types of promoters allowed the production of specific mRNAs and detectable levels of recombinant VP60, which were higher for the constructs carrying the modified 35S promoter. Rabbits immunized with leaf extracts from plants carrying this modified 35S promoter showed high anti-VP60 antibody titers and were fully protected against the hemorrhagic disease. |
| Databáze: | OpenAIRE |
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