Iodination and the Biological Activity of Gastrin

Autor:	Stagg Bh, J. S. Morley, H. Rochman, John M. Temperley
Rok vydání:	1970
Předmět:	Multidisciplinary Chemical Phenomena Tetrapeptide Chemistry Chloramines Biological activity Radioimmunoassay Rats Epitopes Residue (chemistry) Biochemistry In vivo Iodine Isotopes Gastrins Animals Biological Assay Dimethyl Sulfoxide Specific activity Amino Acid Sequence Iodine Gastrin Hormone
Zdroj:	Nature. 228:58-59
ISSN:	1476-4687 0028-0836
DOI:	10.1038/228058a0
Popis:	RADIOIODINATED polypeptide hormones are widely used as tracers for both radioimmunoassay and in vivo metabolic studies. The process of iodination may induce gross chemical changes which are detectable by physico-chemical techniques, but such techniques may not reveal more subtle changes reflected only in decreased biological activity. Such a situation arises with gastrin, in which iodination of the synthetic hormone by the chloramine-T method1 results in almost total loss of biological activity. This is due to oxidation of the methionyl residue at position 15, which is in the biologically active C-terminal tetrapeptide sequence, and can be prevented by modifying the iodination procedure. The resulting iodinated gastrin has full biological activity, is labelled to the same specific activity as in the original method, and has an increased affinity for antibodies to the hormone.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aec0694ad4587e56c4d85c148fc15828 https://doi.org/10.1038/228058a0 Zobrazit plný text záznamu