Elevated circulatingadenosine deaminase activity in women with preeclampsia: association with pro-inflammatory cytokine production and uric acid levels

Autor: Maria Terezinha Serrão Peraçoli, Vera Borges, Camila F. Bannwart-Castro, José Carlos Peraçoli, Mariana Romao-Veiga, Steven S. Witkin, Maria Salete Sartori, Vanessa S. Giorgi
Přispěvatelé: Universidade Estadual Paulista (Unesp), Weill Cornell Med
Rok vydání: 2016
Předmět:
0301 basic medicine
Adenosine Deaminase
medicine.medical_treatment
Interleukin-1beta
chemistry.chemical_compound
0302 clinical medicine
Adenosine deaminase
Pre-Eclampsia
Pregnancy
IL-1 beta
Hyperuricemia
NF-kB
education.field_of_study
030219 obstetrics & reproductive medicine
biology
NF-kappa B
Obstetrics and Gynecology
Cytokine
Female
medicine.symptom
TNF-alpha
Adult
medicine.medical_specialty
Adolescent
Population
Inflammation
Peripheral blood mononuclear cell
Preeclampsia
03 medical and health sciences
Young Adult
Internal medicine
parasitic diseases
Internal Medicine
medicine
Humans
education
business.industry
Tumor Necrosis Factor-alpha
medicine.disease
Uric Acid
030104 developmental biology
Endocrinology
chemistry
Case-Control Studies
Immunology
biology.protein
Leukocytes
Mononuclear
Uric acid
business
Biomarkers
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 2210-7797
Popis: Made available in DSpace on 2018-11-26T17:15:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01 Objective: Preeclampsia is a specific disorder of human pregnancy that is associated with hyperuricemia and higher levels of pro-inflammatory cytokines. Adenosine deaminase (ADA) is an enzyme present in all human tissues, and is considered an indicator of cellular inflammation. In the present study we assess whether adenosine deaminase (ADA) activity is altered in women with preeclampsia (PE),and contributes to elevated levels of uric acid and pro-inflammatory cytokine production. Study design: The population studied consisted of 60 women with PE, 30 normotensive pregnant women (NT) and 20 non-pregnant women (NP). Uric acid concentration and ADA activity were determined in the serum. Peripheral blood mononuclear cells (PBMCs) were isolated and evaluated for intracellular nuclear transcription factor kappa B (NF-kappa B) levels and for endogenous tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-1 beta) production. The data were evaluated with parametric or non-parametric tests with significance set at P < 0.05. Results: ADA levels were higher in the PE group compared with the NT and NP groups (P < 0.001). A positive correlation between ADA and uric acid levels was identified in women with PE (P < 0.001). Endogenous production of IL-1 beta and TNF-alpha, as well as intracellular NF-kappa B levels, were higher in PBMCs from the PE group than from NT and NP women (P < 0.01) and correlated with the ADA concentration in preeclamptic women (P < 0.01). Conclusion: An elevation in ADA activity in women with PE may contribute to their increased levels of uric acid and pro-inflammatory immune activity. (C) 2016 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved. Sao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Sao Paulo, Brazil Weill Cornell Med, Dept Obstet & Gynecol, New York, NY USA Sao Paulo State Univ, Dept Gynecol & Obstet, Sao Paulo, Brazil Sao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Sao Paulo, Brazil Sao Paulo State Univ, Dept Gynecol & Obstet, Sao Paulo, Brazil
Databáze: OpenAIRE
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