Atypical Transcriptional Activation by TCF via a Zic Transcription Factor in C. elegans Neuronal Precursors
| Autor: | Pauline Mélénec, Magali Iché-Torres, Ute Rothbächer, Sabrina Murgan, Oliver Hobert, Willi Kari, Vincent Bertrand |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Transcriptional Activation
animal structures Models Neurological [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology TCF/LEF family DNA-binding protein Article General Biochemistry Genetics and Molecular Biology Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Transcription (biology) Basic Helix-Loop-Helix Transcription Factors Animals Binding site Caenorhabditis elegans Caenorhabditis elegans Proteins Mode of action Wnt Signaling Pathway Molecular Biology Transcription factor Body Patterning 030304 developmental biology Homeodomain Proteins 0303 health sciences Neuropeptides High Mobility Group Proteins Wnt signaling pathway Cell Biology biology.organism_classification Molecular biology DNA-Binding Proteins TCF Transcription Factors 030217 neurology & neurosurgery Transcription Factors Developmental Biology |
| Zdroj: | Developmental Cell Developmental Cell, 2015, 33 (6), pp.737-45. ⟨10.1016/j.devcel.2015.04.018⟩ Developmental Cell, Elsevier, 2015, 33 (6), pp.737-45. ⟨10.1016/j.devcel.2015.04.018⟩ |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2015.04.018⟩ |
| Popis: | International audience; Transcription factors of the TCF family are key mediators of the Wnt/β-catenin pathway. TCF usually activates transcription on cis-regulatory elements containing TCF binding sites when the pathway is active and represses transcription when the pathway is inactive. However, some direct targets display an opposite regulation (activated by TCF in the absence of Wnt), but the mechanism behind this atypical regulation remains poorly characterized. Here, we use the cis-regulatory region of an opposite target gene, ttx-3, to dissect the mechanism of this atypical regulation. Using a combination of genetic, molecular, and biochemical experiments, we establish that, in the absence of Wnt pathway activation, TCF activates ttx-3 expression via a Zic binding site by forming a complex with a Zic transcription factor. This mechanism is later reinforced by specific bHLH factors. This study reveals an atypical mode of action for TCF that may apply to other binary decisions mediated by Wnt signaling. |
| Databáze: | OpenAIRE |
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