Characterization of the Mycoplasma hominis ftsZ gene and its sequence variability in mycoplasma clinical isolates
| Autor: | O. V. Smirnova, S.N. Borchsenius, J.A Ayala, V.N Lazyrev, T. A. Akopian, Kuvat Momynaliev, V. V. Chelysheva, A. N. Simankova, Vadim M. Govorun |
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| Rok vydání: | 2002 |
| Předmět: |
Molecular Sequence Data
Biophysics macromolecular substances Mycoplasma hominis medicine.disease_cause physiological processes Biochemistry Microbiology GTP Phosphohydrolases Plasmid Bacterial Proteins Gene expression medicine Escherichia coli Amino Acid Sequence Cloning Molecular FtsZ Molecular Biology Gene Genetics biology Base Sequence Sequence Homology Amino Acid Chromosome Mapping Genetic Variation Cell Biology Mycoplasma Chromosomes Bacterial biology.organism_classification Recombinant Proteins Cytoskeletal Proteins Genes Bacterial biology.protein bacteria biological phenomena cell phenomena and immunity Mycoplasma genitalium Sequence Alignment |
| Zdroj: | Biochemical and biophysical research communications. 293(1) |
| ISSN: | 0006-291X |
| Popis: | We cloned and sequenced Mycoplasma hominis chromosomal fragment containing ftsZ gene. The wild-type expression of the gene was shown at RNA level by reverse transcription followed by PCR amplification. We revealed that M. hominis FtsZ had a comparatively low similarity to proteins of Mycoplasma genitalium and Mycoplasma pneumoniae. After full ftsZ gene sequencing for 14 clinical isolates of M. hominis, single-nucleotide substitutions were found in 21 positions, 6 of them being common for almost all isolates. This ftsZ gene polymorphism may be used for subtyping of M. hominis in clinical samples. Expression of the M. hominis ftsZ gene in different Escherichia coli strains was also demonstrated, and M. hominis FtsZ protein was purified from E. coli cells transformed with recombinant expression plasmid. Complementation between the M. hominis FtsZ and E. coli FtsZ could be shown. The comparison of FtsZ protein structures may also be used for investigation of bacterial phylogenetic relationships. |
| Databáze: | OpenAIRE |
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