HTLV-III antibody testing in three Danish blood banks
| Autor: | N. Grunnet, Casper Jersild, B. Ørskov Lindhardt, Ebbe Dickmeiss, Lars P. Ryder, Henrik Toft Sørensen, K. Ulrich, Johannes Ravn Jørgensen |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
biology
business.industry Denmark HTLV-III Antibody Blood Donors Enzyme-Linked Immunosorbent Assay Hematology General Medicine Optical density Antibodies Viral Deltaretrovirus language.human_language Danish Specific antibody HLA Antigens Immunology biology.protein language Medicine Blood Banks Humans Hla antibodies Dual wavelength Antibody business |
| Zdroj: | Vox sanguinis. 51 |
| ISSN: | 0042-9007 |
| Popis: | The Organon Teknika Vironostika anti-HTLV-III/LAV test was evaluated in three Danish blood banks. The evaluation comprised in total 3,940 consecutive donors. In all three blood banks the tests were carried out exactly according to the manufacturer's instructions, using a low cut-off value defined as (4N + P)/5, where N and P are means of optical densities of known negative and positive samples. By this method the overall frequency of repeatably positive samples was 0.30%. When tested by Western blot, however, none of these samples were shown to contain specific antibodies against HTLV-III/LAV proteins. When testing different categories of patients, only sera containing HLA antibodies gave rise to false-positive reactions. Finally, important differences in the results were observed regarding sample preparation, single or dual wavelength optical density readings, and the experience of the technical staff. |
| Databáze: | OpenAIRE |
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