Novel CNS Glutamate Receptor Subunit Genes of Drosophila melanogaster
| Autor: | Bertram Schmitt, Bettina Lenz-Böhme, Heinrich Betz, Michaela Völkner |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Central Nervous System
DNA Complementary Embryo Nonmammalian Sequence analysis Protein subunit Immunoblotting Molecular Sequence Data Sequence alignment Biochemistry Cellular and Molecular Neuroscience Drosophilidae Complementary DNA Animals 5-HT5A receptor Fluorescent Antibody Technique Indirect X chromosome Genetics Sequence Homology Amino Acid biology Sequence Analysis DNA biology.organism_classification Cell biology Drosophila melanogaster Receptors Glutamate Sequence Alignment |
| Zdroj: | Journal of Neurochemistry. 75:1791-1799 |
| ISSN: | 0022-3042 |
| DOI: | 10.1046/j.1471-4159.2000.0751791.x |
| Popis: | We report the identification and characterization of two genes from Drosophila melanogaster that encode novel ionotropic glutamate receptor proteins, named DGluR-IB and DNMDAR-II, and that are located on chromosome 3L, region 67AB, and the X chromosome, position 2B, respectively. The DGluR-IB full-length cDNA was isolated from Drosophila embryonic and head libraries. The encoded protein of 1,095 amino acids displays high sequence identity (73%) to DGluR-IA. The DNMDAR-II gene was identified by sequence-homology searches in databases. The deduced protein shows moderate sequence identity (29-31%) to the mouse NMDAR2A-D receptor subunits. Whole-mount in situ hybridization on embryos revealed DGluR-IB and DNMDAR-II transcripts in the CNS. Immunofluorescence analysis of the adult fly brain indicates that the DGluR-IB protein is expressed in neurons implicated in the regulation of the circadian clock. |
| Databáze: | OpenAIRE |
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