Multiple Correspondence Analysis on Amino Acid Properties within the Variable Region of the Capsid Protein Shows Differences between Classical and Virulent Systemic Feline Calicivirus Strains
| Autor: | Cecile Sigoillot-Claude, Hervé Poulet, Sylvie Brunet, Daniel Pialot |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Models Molecular congenital hereditary and neonatal diseases and abnormalities 040301 veterinary sciences viruses Virulence Biology Cat Diseases phylogeny Virus Article Disease Outbreaks 0403 veterinary science Pathogenesis 03 medical and health sciences Virology Feline Calicivirus Animals Amino Acid Sequence Pathogen Gene Tropism Caliciviridae Infections multivariate statistical analysis Feline calicivirus viral systemic disease 04 agricultural and veterinary sciences VP1 sequence biology.organism_classification JAM-1 030104 developmental biology Infectious Diseases Capsid Multivariate Analysis Cats pathotypes Capsid Proteins Cell Adhesion Molecules Sequence Alignment Calicivirus Feline |
| Zdroj: | Viruses Volume 11 Issue 12 |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v11121090 |
| Popis: | Feline calicivirus (FCV) is a widespread and highly prevalent pathogen of domestic cats, responsible for mild upper respiratory tract disease. Outbreaks of severe virulent systemic disease (VSD) associated with FCV infection have been reported worldwide. VSD FCV strains have a broader tropism and cause a systemic vascular compromise. Despite clear differences in the pathogenesis of VSD and oral respiratory infections, attempts to identify specific molecular markers of VSD strains on the major capsid protein VP1 have failed. Region E of VP1 is responsible for the interaction with the cell receptor Junctional Adhesion Molecule JAM-1 (FeJAM-1) and with VP2 minor capsid protein during the entry of the virus. We carried out an original analysis on the sequences from region E of VSD and classical strains. A Multiple Correspondence Analysis was performed on a Boolean matrix built by coding sequences on the basis of their amino acid properties. For the first time, this approach was able to differentiate VSD and classical FCV. Seven remarkable residue positions were shown to be statistically significant for pathotype differentiation, mainly located in the N-terminal hypervariable part of region E. As structural analysis suggested an interaction of these residues with FeJAM-1 or VP2, post-binding events, and specific conformational changes may explain the difference of pathogenesis between pathotypes. |
| Databáze: | OpenAIRE |
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