Dynamic light scattering biosensing based on analyte-induced inhibition of nanoparticle aggregation
| Autor: | K. G. Shevchenko, V. R. Cherkasov, A. Ringaci, Alexander D. Levin, Petr I. Nikitin, E. B. Drozhzhennikova, Maxim P. Nikitin, Mikhail K. Alenichev |
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| Rok vydání: | 2020 |
| Předmět: |
Analyte
Materials science Orders of magnitude (temperature) Metal Nanoparticles Nanoparticle Biosensing Techniques 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Dynamic light scattering Limit of Detection Wide dynamic range Animals Magnetite Nanoparticles Immunoassay Chromatography 010401 analytical chemistry 021001 nanoscience & nanotechnology Dynamic Light Scattering Anti-Bacterial Agents 0104 chemical sciences Chloramphenicol Milk Colloidal gold Magnetic nanoparticles Gold 0210 nano-technology Antibodies Immobilized Biosensor Food Analysis |
| Zdroj: | Analytical and Bioanalytical Chemistry. 412:3423-3431 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | A new approach to direct quantitative detection of small molecules (haptens) by dynamic light scattering biosensing is presented. The proposed technique implements a homogeneous competitive immunoassay and is based on optical detection of specific inhibition of nanoparticle aggregation induced by the analyte in a sample. The technique performance was tested both in buffer and milk for detection of chloramphenicol - antibiotic relevant to food safety diagnostics. Good specificity, sensitivity (LOD in milk is 2.4 ng/ml), precision (4.0 ± 1.2%), ruggedness (8.3%), and 96% recovery in conjunction with a record wide dynamic range (3 orders of magnitude) of the nanosensing technique were demonstrated. Such characteristics complemented by the assay simplicity (no washing step) and a short assay time make the approach attractive for application as an analytical platform for point-of-care and field-oriented diagnostics. Graphical abstract. |
| Databáze: | OpenAIRE |
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