Temperature-Sensitive Gold Nanoparticle-Coated Pluronic-PLL Nanoparticles for Drug Delivery and Chemo-Photothermal Therapy
| Autor: | Jianhua Chen, Baoshan Han, Ying Sun, Ming Shen, Lei Liu, Jin Li, Yourong Duan, Qi Wang, Li Ding |
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| Rok vydání: | 2016 |
| Předmět: |
Biocompatibility
Paclitaxel Cell Survival Pluronic-b-poly(L-lysine) Medicine (miscellaneous) Nanoparticle Metal Nanoparticles Mice Nude Biocompatible Materials 02 engineering and technology Poloxamer 010402 general chemistry 01 natural sciences Mice Drug Delivery Systems Dynamic light scattering In vivo Cell Line Tumor Animals Humans Thermally Sensitive Polylysine Cytotoxicity Pharmacology Toxicology and Pharmaceutics (miscellaneous) One-Pot Synthesis Micelles Drug Carriers Mice Inbred BALB C Chemistry technology industry and agriculture Temperature Photothermal therapy 021001 nanoscience & nanotechnology Fluoresceins Chemo-Photothermal 0104 chemical sciences Gold Nanoparticle Drug delivery Biophysics Female Gold Rabbits 0210 nano-technology Drug Delivery Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| Popis: | Gold nanoparticle-coated Pluronic-b-poly(L-lysine) nanoparticles (Pluronic-PLL@Au NPs) were synthesized via an easy one-step method and employed as carriers for the delivery of paclitaxel (PTX) in chemo-photothermal therapy, in which Pluronic-PLL acts as the reductant for the formation of AuNPs without the need for an additional reducing agent. Methods The deposition of AuNPs on the surface of Pluronic-PLL micelles and the thermal response of the system were followed via ultraviolet-visible spectroscopy and dynamic light scattering. Calcein-AM and MTT assays were used to study the cell viability of MDA-MB-231 cells treated with PTX-loaded Pluronic-PLL@Au NPs, and we then irradiated the cells with NIR light. Results An obvious temperature response was observed for the Pluronic-PLL@Au NPs. Blood compatibility and in vitro cytotoxicity assays confirmed that the Pluronic-PLL@Au NPs have excellent biocompatibility. Compared to Taxol, the PTX-loaded Pluronic-PLL@Au NPs exhibited higher cytotoxicity in MDA-MB-231 cells. All of these results and confocal laser scanning microscopy analysis results suggest that Pluronic-PLL@Au NPs greatly enhance the cellular uptake efficiency of the drug. Conclusion As confirmed by in vitro and in vivo studies, the combination of chemotherapy and photothermal therapy can cause more damage than chemo- or photothermal therapy did alone, demonstrating the synergistic effect of chemo-photothermal treatment. Thus, the as-prepared Pluronic-PLL@Au NPs are promising for chemo-photothermal therapy. |
| Databáze: | OpenAIRE |
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