Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules
| Autor: | Haruna Yoshimura, Takumi Higaki, Masaki Hatano, Masayuki Igarashi, Tokio Tani, Takashi Ishida, Shinichiro Sawa, Masatsugu Takekawa, Hayato Ishikawa, Takashi Ideue |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death Plant molecular biology Streptomyces werraensis Science Organic chemistry Plant Development Plant cell biology Microtubules 01 natural sciences Article HeLa Structure-Activity Relationship 03 medical and health sciences Microtubule Plant Cells Mode of action Cytoskeleton Actin Natural products Biological Products Multidisciplinary Molecular Structure biology Herbicides Cell growth Chemistry Total synthesis Plants biology.organism_classification Actins Streptomyces 030104 developmental biology Biochemistry Medicine 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The discovery and useful application of natural products can help improve human life. Chemicals that inhibit plant growth are broadly utilized as herbicides to control weeds. As various types of herbicides are required, the identification of compounds with novel modes of action is desirable. In the present study, we discovered a novel N-alkoxypyrrole compound, kumamonamide from Streptomyces werraensis MK493-CF1 and established a total synthesis procedure. Resulted in the bioactivity assays, we found that kumamonamic acid, a synthetic intermediate of kumamonamide, is a potential plant growth inhibitor. Further, we developed various derivatives of kumamonamic acid, including a kumamonamic acid nonyloxy derivative (KAND), which displayed high herbicidal activity without adverse effects on HeLa cell growth. We also detected that kumamonamic acid derivatives disturb plant microtubules; and additionally, that KAND affected actin filaments and induced cell death. These multifaceted effects differ from those of known microtubule inhibitors, suggesting a novel mode of action of kumamonamic acid, which represents an important lead for the development of new herbicides. |
| Databáze: | OpenAIRE |
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