Performance of the ImmuView and BinaxNOW assays for the detection of urine and cerebrospinal fluid Streptococcus pneumoniae and Legionella pneumophila serogroup 1 antigen in patients with Legionnaires' disease or pneumococcal pneumonia and meningitis

Autor: Leslie A Wolf, Charlotte Sværke Jørgensen, Paul H. Edelstein
Přispěvatelé: Edelstein, Paul H [0000-0002-4069-5279], Apollo - University of Cambridge Repository
Rok vydání: 2020
Předmět:
Male
Pulmonology
Physiology
Urine
Pathology and Laboratory Medicine
medicine.disease_cause
Nervous System
Pediatrics
Legionella pneumophila
Medical Conditions
Infectious Diseases of the Nervous System
Medicine and Health Sciences
Medicine
Child
Cerebrospinal Fluid
Multidisciplinary
Vaporization
biology
Physics
Pneumococcus
Condensed Matter Physics
Body Fluids
Bacterial Pathogens
Infectious Diseases
Streptococcus pneumoniae
Neurology
Medical Microbiology
Child
Preschool
Physical Sciences
Pneumococcal pneumonia
Boiling
Legionnaires' disease
Biological Assay
Female
Anatomy
Pathogens
Legionnaires' Disease
Phase Transitions
Meningitis
Research Article
Adult
Adolescent
Legionella
Science
Inflammatory Diseases
Immunologic Tests
Serogroup
Microbiology
Sensitivity and Specificity
Young Adult
Antigen
Humans
Microbial Pathogens
Antigens
Bacterial
Bacteria
business.industry
Organisms
Biology and Life Sciences
Streptococcus
Infant
Pneumonia
Pneumonia
Pneumococcal
biology.organism_classification
medicine.disease
business
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 8, p e0238479 (2020)
Popis: The performances of the ImmuView Streptococcus pneumoniae (Sp) and Legionella pneumophila (Lp) urinary antigen test were compared to that of the BinaxNOW Sp and Lp assays, using frozen urine from 166 patients with Legionnaires' disease (LD) and 59 patients with pneumococcal pneumonia. Thirty Sp-positive or contrived cerebrospinal fluids (CSF) were also tested. Test specimens were collected and tested at different sites, with each site testing unique specimens by technologists blinded to expected results. No significant differences in test concordances were detected for the ImmuView and BinaxNOW assays for the Sp or Lp targets for urine from patients with pneumococcal pneumonia or LD when performance from both sites were combined. At one of two test sites the ImmuView Lp assay was more sensitive than the BinaxNOW assay, with no correlation between test performance and Lp serogroup 1 monoclonal type. Urines from six of seven patients with LD caused by Legionella spp. bacteria other than Lp serogroup 1 were negative in both assays. Both tests had equivalent performance for Sp-positive CSF. The clinical sensitivities for pneumococcal pneumonia were 88.1 and 94.4% for the ImmuView and Binax assays, and 87.6 and 84.2% for the Lp assays, respectively. Test specificities for pneumococcal pneumonia were 96.2 and 97.0% for the ImmuView and Binax assays, and 99.6 and 99.1% for the Lp assays. Both assays were highly specific for Sp in pediatric urines from children with nasopharyngeal colonization by the bacterium. ImmuView and BinaxNOW assay performance was equivalent in these studies.
Databáze: OpenAIRE
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