Dental epithelial histo-morphogenesis in the mouse: positional information versus cell history

Autor: Amal Nadiri, Fabienne Perrin-Schmitt, Sabine Bopp-Kuchler, Hervé Lesot, Songlin Wang, Bing Hu
Přispěvatelé: Biomatériaux : Processus biophysiques et biologiques aux interfaces, Université Louis Pasteur - Strasbourg I-Faculté de Chirurgie Dentaire-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2005
Předmět:
Pathology
medicine.medical_specialty
Mesenchyme
Cell Culture Techniques
Cell Separation
Biology
MESH: Cell Separation
MESH: Enamel Organ
Mesoderm
Mice
03 medical and health sciences
0302 clinical medicine
stomatognathic system
medicine
Animals
MESH: Animals
MESH: Mice
Inbred ICR
MESH: Mice
General Dentistry
Reduced enamel epithelium
030304 developmental biology
MESH: Cell Culture Techniques
MESH: Mesoderm
Mice
Inbred ICR
0303 health sciences
Inner enamel epithelium
Outer enamel epithelium
Enamel Organ
Enamel organ
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Epithelial Cells
030206 dentistry
Cell Biology
General Medicine
Amelogenesis
Molar
Enamel knot
Cell biology
stomatognathic diseases
medicine.anatomical_structure
Otorhinolaryngology
MESH: Epithelial Cells
Odontogenesis
MESH: Molar
Ameloblast
MESH: Odontogenesis
Zdroj: Archives of Oral Biology
Archives of Oral Biology, Elsevier, 2005, 50 (2), pp.131-6. ⟨10.1016/j.archoralbio.2004.09.007⟩
ISSN: 0003-9969
DOI: 10.1016/j.archoralbio.2004.09.007
Popis: International audience; Reciprocal epithelial-mesenchymal interactions control odontogenesis and the cap stage tooth germ mesenchyme specifies crown morphogenesis. The aim of this work was to determine whether this mesenchyme could also control epithelial histogenesis. Dental mesenchyme and enamel organ were dissociated from mouse first lower molars at E14. At this early cap stage, the enamel organ consists of four cell types forming the inner dental epithelium (IDE), primary enamel knot (PEK), outer dental epithelium (ODE) and the stellate reticulum (SR). Pelleted trypsin-dissociated single dental epithelial cells, which had lost all positional information, were reassociated to either dental mesenchyme or dissociated mesenchymal cells and cultured in vitro. Although with different timings, teeth developed in both types of experiments showing a characteristic dental epithelial histogenesis, cusp formation, and the differentiation of functional odontoblasts and ameloblasts. The rapid progression of the initial steps of histogenesis suggested that the cell history was not memorized. The dental mesenchyme, as well as dissociated mesenchymal cells, induced the formation of a PEK indicating that no specific organisation in the mesenchyme is required for this step. However, the proportion of well-formed multicusped teeth was much higher when intact mesenchyme was used instead of dissociated mesenchymal cells. The mesenchymal cell dissociation had consequences for the functionality of the newly-formed PEK.
Databáze: OpenAIRE
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