Characterization of an extended-spectrum class A β-lactamase from a novel enterobacterial species taxonomically related to Rahnella spp./Ewingella spp

Autor: Marie-Frédérique Lartigue, Gaelle Cuzon, Mikhail V. Edelstein, Laurent Poirel, Patrice Nordmann, Sylvain Brisse
Přispěvatelé: Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Résistances émergentes aux antibiotiques, Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de médecine, Université Paris-Sud - Paris 11 (UP11), Smolensk State Medical Academy, Partenaires INRAE, Institut Pasteur [Paris], European Community HEALTH-F3-2001-278232, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Université Paris-Sud - Paris 11 - Faculté de médecine (UP11 UFR Médecine), Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris] (IP), This work was partially funded by a grant from the INSERM (UMR914), by the Université Paris-Sud, France, and by grants from the European Community (R-GNOSIS, FP7/HEALTH-F3-2011-282512 and MAGIC-BULLET, FP7/HEALTH-F3-2001-278232)., European Project: 278232,EC:FP7:HEALTH,FP7-HEALTH-2011-two-stage,MAGICBULLET(2012), European Project: 282512,EC:FP7:HEALTH,FP7-HEALTH-2011-single-stage,R-GNOSIS(2011), ProdInra, Migration, Optimisation of treatment with off-patent antimicrobial agents of ventilator-associated pneumonia (VAP) - MAGICBULLET - - EC:FP7:HEALTH2012-01-01 - 2015-12-31 - 278232 - VALID, Resistance in Gram-Negative Organisms: Studying Intervention Strategies - R-GNOSIS - - EC:FP7:HEALTH2011-10-01 - 2017-03-31 - 282512 - VALID
Rok vydání: 2013
Předmět:
MESH: Sequence Analysis
DNA
Cefotaxime
MESH: Sequence Homology
Amino Acid
Gene Expression
MESH: beta-Lactamases
medicine.disease_cause
extended-spectrum β-lactamases
Plasmid
MESH: Spectrophotometry
Ultraviolet
polycyclic compounds
Pharmacology (medical)
Cloning
Molecular
CTX-M
MESH: Enterobacteriaceae Infections
chemistry.chemical_classification
0303 health sciences
biology
MESH: Escherichia coli
Hydrolysis
Enterobacteriaceae Infections
MESH: Cefotaxime
Chromatography
Ion Exchange
extended-spectrum b-lactamases
Amino acid
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infectious Diseases
Ewingella americana
MESH: Hydrolysis
medicine.drug
DNA
Bacterial
Microbiology (medical)
MESH: Gene Expression
Molecular Sequence Data
Penicillins
Serratia
beta-Lactamases
Microbiology
Gram-negatives
MESH: Enterobacteriaceae
03 medical and health sciences
Enterobacteriaceae
MESH: Penicillins
Escherichia coli
medicine
Humans
MESH: Cloning
Molecular
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Gene
030304 developmental biology
Pharmacology
MESH: Molecular Sequence Data
MESH: Humans
Sequence Homology
Amino Acid
030306 microbiology
Sequence Analysis
DNA
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
MESH: Chromatography
Ion Exchange
MESH: DNA
Bacterial
chemistry
Spectrophotometry
Ultraviolet
Rahnella aquatilis
Zdroj: Journal of Antimicrobial Chemotherapy
Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2013, 68 (8), pp.1733-1736. ⟨10.1093/jac/dkt122⟩
Journal of Antimicrobial Chemotherapy, 2013, 68 (8), pp.1733-1736. ⟨10.1093/jac/dkt122⟩
Journal of Antimicrobial Chemotherapy; Vol 68
ISSN: 1460-2091
0305-7453
DOI: 10.1093/jac/dkt122
Popis: International audience; OBJECTIVES: To characterize the naturally occurring beta-lactamase gene identified from a clinical isolate belonging to a novel enterobacterial species that is closely related to Rahnella spp. and Ewingella spp. METHODS: Shotgun cloning and expression in Escherichia coli were performed in order to characterize this resistance determinant. Enzymatic activities were measured by UV spectrophotometry after an ion-exchange chromatography purification procedure. RESULTS: A chromosomal gene coding for the extended-spectrum beta-lactamase (ESBL) SMO-1 was identified from a novel enterobacterial species that is taxonomically related to Rahnella aquatilis and Ewingella americana. The beta-lactamase efficiently hydrolysed penicillins and cefotaxime, and shared 75% amino acid identity with the plasmid-mediated beta-lactamase SFO-1 from Serratia fonticola, 74% amino acid identity with the plasmid-mediated ESBL CTX-M-2 originating from Kluyvera spp. and 72% amino acid identity with the chromosomally encoded and intrinsic RAHN-1 from R. aquatilis. CONCLUSIONS: We have identified a novel enterobacterial species recovered from a clinical specimen, constituting another potential source of acquired ESBL. The ESBL shared significant similarities with the CTX-M-type enzymes.
Databáze: OpenAIRE
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