Human herpesvirus 6 infection in autologous bone marrow transplant recipients: A prospective study
| Autor: | Pier Egisto Valensin, Francesco Lauria, Giulia Scalia, Monica Tozzi, Donatella Moschettini, Daniele Laszlo, Piero Galieni, D. Donati |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Herpesvirus 6 Human viruses medicine.medical_treatment Antibodies Viral medicine.disease_cause Immunofluorescence Polymerase Chain Reaction Transplantation Autologous Peripheral blood mononuclear cell Herpesviridae Virology medicine Humans Prospective Studies Bone Marrow Transplantation biology medicine.diagnostic_test virus diseases Immunosuppression Herpesviridae Infections Middle Aged biochemical phenomena metabolism and nutrition biology.organism_classification Transplantation Infectious Diseases medicine.anatomical_structure DNA Viral Immunology Leukocytes Mononuclear biology.protein Female Virus Activation Human herpesvirus 6 Bone marrow Antibody |
| Zdroj: | Journal of Medical Virology. 60:39-42 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/(sici)1096-9071(200001)60:1<39::aid-jmv7>3.0.co;2-i |
| Popis: | After primary infection in early life, human herpesvirus 6 (HHV-6) remains latent in the body and may reactivate in subjects with poor immune status. A 180-day longitudinal study of HHV-6 infection was carried out in 23 autologous bone marrow transplant recipients to evaluate reactivation of HHV-6; two of these patients underwent a double transplant. The patients were monitored prospectively for HHV-6 DNA in peripheral blood mononuclear cells (PBMC) by hot start nested PCR. Positive samples were typed by the enzymatic restriction protocol. Positive plasma samples were also tested for HHV-6 DNA. Antibodies against HHV-6 were measured by immunofluorescence. Five and two out of 23 patients had intermittent and persistent positivity to HHV-6 DNA in PBMCs, respectively; four patients carried variant B, and the other three patients both A and B. None of the respective plasma samples were positive. Two patients were positive for HHV-6 antibodies. Since the significance of HHV-6 DNA in PBMCs is unclear, these findings do not necessarily indicate active infection but may be due to mild immunosuppression in autologous BMT recipients. |
| Databáze: | OpenAIRE |
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