Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity
| Autor: | Hyun S. Lillehoj, Yeojin Hong, Kyungbaek Lee, Kang-Nyeong Heo, Janggeun Lee, Anh Duc Truong, Yeong Ho Hong, Geun-Bae Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.drug_class
Antibiotics Antimicrobial peptides lcsh:Animal biochemistry Peptide Article Bacterial cell structure Liver-expressed antimicrobial peptide Microbiology Duck medicine lcsh:QP501-801 Peptide sequence lcsh:SF1-1100 chemistry.chemical_classification biology 0402 animal and dairy science Liver-expressed Antimicrobial Peptide 2 (LEAP-2) 04 agricultural and veterinary sciences Antimicrobial biology.organism_classification 040201 dairy & animal science Animal Biotechnology Disulfide Bond chemistry Animal Science and Zoology lcsh:Animal culture Pathogens Antimicrobial Peptides Bacteria Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences, Vol 32, Iss 7, Pp 1052-1061 (2019) Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1976-5517 1011-2367 |
| DOI: | 10.5713/ajas.18.0571 |
| Popis: | Objective This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. Methods Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted, and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, quantitative real-time polymerase chain reaction was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity. Then, antimicrobial activity assay and fluorescence microscopic analysis were conducted to demonstrate duck LEAP-2 bactericidal activity. Results The duck LEAP-2 peptide sequence showed high identity with those of other avian species (>85%), as well as more than 55% of identity with mammalian sequences. LEAP-2 mRNA was highly expressed in the liver with duodenum next, and then followed by lung, spleen, bursa and jejunum and was the lowest in the muscle. Both of LEAP-2 peptides efficiently killed bacteria, although the disulfide-type LEAP-2 showed more powerful bactericidal activity. Also, gram-positive bacteria was more susceptible to duck LEAP-2 than gram-negative bacteria. Using microscopy, we confirmed that LEAP-2 peptides could kill bacteria by disrupting the bacterial cell envelope. Conclusion Duck LEAP-2 showed its antimicrobial activity against both gram-positive and gram-negative bacteria. Disulfide bonds were important for the powerful killing effect by disrupting the bacterial cell envelope. Therefore, duck LEAP-2 can be used for effective antibiotics alternatives. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|