Performance of the BD GeneOhm MRSA Achromopeptidase Assay for Real-Time PCR Detection of Methicillin-Resistant Staphylococcus aureus in Nasal Specimens

Autor:	Nathan A. Ledeboer, Stephanie Bouchard, Parul Patel, Tobi Karchmer, Susan Condon, Christine C. Ginocchio, Peibing Qin, Lance R. Peterson
Rok vydání:	2011
Předmět:	Methicillin-Resistant Staphylococcus aureus Microbiology (medical) Micrococcaceae Achromopeptidase Mucous membrane of nose Staphylococcal infections medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Specimen Handling Microbiology Bacterial Proteins medicine Humans Bacteriological Techniques biology Serine Endopeptidases Bacteriology Gold standard (test) Staphylococcal Infections biology.organism_classification medicine.disease Methicillin-resistant Staphylococcus aureus Nasal Mucosa Real-time polymerase chain reaction Molecular Diagnostic Techniques Staphylococcus aureus Reagent Kits Diagnostic
Zdroj:	Journal of Clinical Microbiology. 49:2266-2268
ISSN:	1098-660X 0095-1137
Popis:	We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aeacb44b05e13adcc22feca4a23952dd https://doi.org/10.1128/jcm.02431-10 Zobrazit plný text záznamu