Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and entero-aggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya
| Autor: | Béla Nagy, N. Nógrády, K.S. Ghenghesh, Anna Malik, Mohamed A. Dow, Istvan Toth, M. Herpay |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Diarrhea medicine.drug_class Immunology Antibiotics Virulence Libya Microbial Sensitivity Tests medicine.disease_cause Integron Microbiology Polymerase Chain Reaction Bacterial Adhesion Antibiotic resistance Agglutination Tests medicine Escherichia coli Immunology and Allergy Humans Enteropathogenic Escherichia coli Serotyping Adhesins Bacterial Escherichia coli Infections General Veterinary biology Escherichia coli Proteins Infant General Medicine Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification Virology Enterobacteriaceae Infectious Diseases Child Preschool biology.protein Trans-Activators bacteria medicine.symptom HeLa Cells |
| Zdroj: | Comparative immunology, microbiology and infectious diseases. 29(2-3) |
| ISSN: | 0147-9571 |
| Popis: | A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (enteroaggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae+, EAF+, bfp+) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an enteroaggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC. |
| Databáze: | OpenAIRE |
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