Hepatic Microwave Ablation–Induced Tumor Destruction and Animal End Point Survival Can Be Improved by Suppression of Heat Shock Protein 90
| Autor: | Qunfang Zhou, Ping Liang, Jianping Dou, Hong‐yan Zhai, Jie Yu, Xin-yuan Zhu, Fangyi Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Ablation Techniques
Survival Ganetespib Mice Nude Apoptosis Caspase 3 030218 nuclear medicine & medical imaging Hsp90 inhibitor Mice 03 medical and health sciences Liver Neoplasms Experimental 0302 clinical medicine Nude mouse Animals Medicine Radiology Nuclear Medicine and imaging HSP90 Heat-Shock Proteins Microwaves Cell Proliferation 030219 obstetrics & reproductive medicine Radiological and Ultrasound Technology biology business.industry Microwave ablation Triazoles medicine.disease biology.organism_classification Disease Models Animal Treatment Outcome Hepatocellular carcinoma Cancer research Tumor necrosis factor alpha business |
| Zdroj: | Journal of Ultrasound in Medicine. 39:1223-1232 |
| ISSN: | 1550-9613 0278-4297 |
| DOI: | 10.1002/jum.15212 |
| Popis: | OBJECTIVES To investigate the effect of heat shock protein 90 (HSP90) modulation on tumor necrosis, apoptosis, tumor growth delay, and end point survival by combining microwave ablation (MWA) with an HSP90 inhibitor in a nude mouse model. METHODS This study was approved by the Ethics Committee. Forty mice with HepG2 subcutaneous xenograft tumors (10 ± 1 mm) were randomized into 4 groups: (1) no treatment, (2) MWA only, (3) the HSP90 inhibitor ganetespib only, and (4) ganetespib combined with MWA. Tumors were harvested 24 hours after treatment, and gross coagulation diameters were measured. The effect of ganetespib on HSP90 and caspase 3 expression in the periablational rim was assessed. Another 40 mice with the same tumors and groupings were observed after treatment. Tumor growth curve and Kaplan-Meier survival analyses were performed with a tumor diameter of 2.2 cm and 40 days of survival as the defined survival end points. RESULTS Combination treatment significantly increased the coagulation size compared to tumors treated with MWA or ganetespib alone (P |
| Databáze: | OpenAIRE |
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