Free D-amino acids produced by commensal bacteria in the colonic lumen
| Autor: | Eiichiro Fukusaki, Junko Iida, Hazuki Tamada, Takanari Hattori, Yoshihiro Hayakawa, Shuichi Kawana, Mitsuharu Matsumoto, Akihiro Kunisawa, Shinichi Kawano, Yusuke Kitada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Firmicutes 030106 microbiology Microbial metabolism Lumen (anatomy) lcsh:Medicine Article Microbiology 03 medical and health sciences Mice Tandem Mass Spectrometry Animals Amino Acids Receptor lcsh:Science Colonic lumen chemistry.chemical_classification Mice Inbred ICR Multidisciplinary biology Bacteria Chemistry lcsh:R biology.organism_classification Commensalism Amino acid Gastrointestinal Microbiome Intestines 030104 developmental biology lcsh:Q Chromatography Liquid |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018) |
| ISSN: | 2045-2322 |
| Popis: | D-amino acids (D-AAs) have various biological activities, such as activation of N-methyl-D-aspartic acid (NMDA) receptor as a co-agonist by D-Ser. Since several free D-AAs are released in the broth monocultured with bacterium and D-AAs are probably utilized for bacterial communication, we presume that intestinal microbiota releases several kinds of free D-AAs, which may be involved in the hosts’ health. However, presently, only four free D-AAs have been found in the ceacal lumen, but not in the colonic lumen. Here, we showed, by simultaneous analysis of chiral AAs using high-sensitivity liquid chromatography-tandem mass spectrometry (LC-MS/MS), that 12 free D-AAs (D-Ala, D-Arg, D-Asp, D-Gln, D-Glu, D-allo-Ile, D-Leu, D-Lys, D-Met, D-Phe, D-Ser, and D-Trp) are produced by intestinal microbiota and identified bacterial groups belonging to Firmicutes as the relevant bacterial candidates. |
| Databáze: | OpenAIRE |
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