| Autor: |
Liu, Xuemeng, Hu, Yaotian, Xue, Zhiyi, Zhang, Xun, Liu, Xiaofei, Liu, Guowei, Wen, Muzi, Chen, Anjing, Huang, Bin, Li, Xingang, Yang, Ning, Wang, Jian |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22612796 |
| Popis: |
Additional file 1: Figure S1. Valtrate inhibits cell proliferation in GBM cells. Figure S2. Valtrate promotes apoptosis in GBM cells via the mitochondrial pathway. Figure S3. Valtrate suppresses migration and invasion of GBM cells. Figure S4. PDGFRA is a potential target downregulated by valtrate in GBM cells. (A) Volcano plot showing the up- and downregulated genes, red and blue colors, respectively, obtained from RNA-seq analysis. Cells were treated with valtrate (U251: 2 μM, GBM#P3: 0.5 μM) for 48 h and RNA was isolated and sequenced. (B) Cell viability of LN229-PDGFRA-OE under the conditions indicated as determined with the CCK-8 assay. (C) Representative images of EdU assays for U251- and LN229-PDGFRA-OE cells under the conditions indicated. Scale bar, 50 μm. (D) Flow cytometry to detect the percentage of apoptotic U251- and GBM#P3-PDGFRA-OE cells under the conditions indicated as determined with annexin V-FITC and PI staining. (E) Representative images of 3D invasion assay for U251- and GBM#P3-PDGFRA-OE PDGFRA cells under the conditions indicated, with or without valtrate. Scale bar, 200 μm. All data are expressed as the mean ± SD of values from triplicate experiments and the differences between groups were analyzed with the Student’s t-test. *p < 0.05. Figure S5. Valtrate elicits anti-GBM activity through inhibition of the PDGFRA/MEK/ERK signaling pathway. Figure S6. Valtrate exerts its antitumor effects in vivo. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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