Production, structure and morphology of exopolysaccharides yielded by submerged fermentation of Antrodia cinnamomea
| Autor: | Bo-Bo Zhang, Lei Chen, Yuge Niu, Liming Liu, Ge Mengdie, Hui Suan Ng, Zheng-Qi Wang |
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| Rok vydání: | 2018 |
| Předmět: |
Morphology (linguistics)
Polymers and Plastics chemistry.chemical_element 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Polysaccharides Materials Chemistry Yeast extract Food science Organic Chemistry 021001 nanoscience & nanotechnology Nitrogen 0104 chemical sciences Culture Media chemistry Galactose Yield (chemistry) Antrodia Fermentation 0210 nano-technology Sugars Carbon Antrodia cinnamomea Macromolecule |
| Zdroj: | Carbohydrate polymers. 205 |
| ISSN: | 1879-1344 |
| Popis: | Carbon and nitrogen sources in culture medium of Antrodia cinnamomea were optimized to eliminate the interference of exterior macromolecules on exopolysaccharide (EPS) yield by submerged fermentation. The results suggested that culture medium containing 50 g/L of glucose and 20 g/L of yeast extract as the optimal carbon and nitrogen sources could produce 1.03 g/L of exopolysaccharides. After purification, two heteropolysaccharides (AC-EPS1 and AC-EPS2) were obtained and characterized to provide the basic structure information. As the main component of the produced EPS, AC-EPS2 (accounting for 89.63%) was mainly composed of galactose (87.42%) with Mw (molecular weight) and R.M.S. (root-mean-square) radius of 1.18 × 105 g/mol and 25.3 nm, respectively. Furthermore, the spherical and flexible chain morphologies of EPS were observed in different solvents by TEM. The structural and morphological information of purified EPS were significant for further study on their structure-activity relationship and related applications. |
| Databáze: | OpenAIRE |
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