Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics
| Autor: | Ashutosh Chilkoti, L.A. Klumb, Oliver W. Press, Philip Tan, Charles Cheung, Chantal Lackey, Niren Murthy, Allan S. Hoffman, Patrick S. Stayton, F.Scott Wilbur |
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| Rok vydání: | 2000 |
| Předmět: |
Streptavidin
Polymers Protein Conformation Genetic enhancement Pharmaceutical Science Nanotechnology Genetic Therapy Protein engineering Computational biology Biology Protein Engineering Smart polymer Antibodies Molecular engineering chemistry.chemical_compound Drug Delivery Systems chemistry Indicators and Reagents Drug carrier Intracellular Function (biology) |
| Zdroj: | Journal of Controlled Release. 65:203-220 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/s0168-3659(99)00236-9 |
| Popis: | There are many protein and DNA based therapeutics under development in the biotechnology and pharmaceutical industries. Key delivery challenges remain before many of these biomolecular therapeutics reach the clinic. Two important barriers are the effective targeting of drugs to specific tissues and cells and the subsequent intracellular delivery to appropriate cellular compartments. In this review, we summarize protein engineering work aimed at improving the stability and refolding efficiency of antibody fragments used in targeting, and at constructing new streptavidin variants which may offer improved performance in pre-targeting delivery strategies. In addition, we review recent work with pH-responsive polymers that mimic the membrane disruptive properties of viruses and toxins. These polymers could serve as alternatives to fusogenic peptides in gene therapy formulations and to enhance the intracellular delivery of protein therapeutics that function in the cytoplasm. |
| Databáze: | OpenAIRE |
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