Glycomics Profiling of Chinese Hamster Ovary Cell Glycosylation Mutants Reveals N-Glycans of a Novel Size and Complexity
| Autor: | Anne Dell, Pamela Stanley, Hung Hsiang Huang, Simon J. North, A. Tony Etienne, Alana Trollope, Stuart M. Haslam, Subha Sundaram, Sara Chalabi, Jihye Jang-Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Glycan
Glycosylation Mutant Glycobiology and Extracellular Matrices CHO Cells Biology Biochemistry law.invention Glycomics 03 medical and health sciences chemistry.chemical_compound Lec Mutants Cricetulus Methods/Mass Spectrometry law Polysaccharides Cricetinae Glycosyltransferase Animals Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chinese hamster ovary cell 030302 biochemistry & molecular biology Cell Biology carbohydrates (lipids) chemistry Carbohydrate Sequence Poly-N-acetyllactosamine Mutation Recombinant DNA biology.protein Glycoprotein |
| Zdroj: | The Journal of Biological Chemistry |
| Popis: | Identifying biological roles for mammalian glycans and the pathways by which they are synthesized has been greatly facilitated by investigations of glycosylation mutants of cultured cell lines and model organisms. Chinese hamster ovary (CHO) glycosylation mutants isolated on the basis of their lectin resistance have been particularly useful for glycosylation engineering of recombinant glycoproteins. To further enhance the application of these mutants, and to obtain insights into the effects of altering one specific glycosyltransferase or glycosylation activity on the overall expression of cellular glycans, an analysis of the N-glycans and major O-glycans of a panel of CHO mutants was performed using glycomic analyses anchored by matrix-assisted laser desorption ionization-time of flight/time of flight mass spectrometry. We report here the complement of the major N-glycans and O-glycans present in nine distinct CHO glycosylation mutants. Parent CHO cells grown in monolayer versus suspension culture had similar profiles of N- and O-GalNAc glycans, although the profiles of glycosylation mutants Lec1, Lec2, Lec3.2.8.1, Lec4, LEC10, LEC11, LEC12, Lec13, and LEC30 were consistent with available genetic and biochemical data. However, the complexity of the range of N-glycans observed was unexpected. Several of the complex N-glycan profiles contained structures of m/z approximately 13,000 representing complex N-glycans with a total of 26 N-acetyllactosamine (Gal beta1-4GlcNAc)(n) units. Importantly, the LEC11, LEC12, and LEC30 CHO mutants exhibited unique complements of fucosylated complex N-glycans terminating in Lewis(x) and sialyl-Lewis(x) determinants. This analysis reveals the larger-than-expected complexity of N-glycans in CHO cell mutants that may be used in a broad variety of functional glycomics studies and for making recombinant glycoproteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|