Inducible serum resistance in Salmonella typhimurium is dependent on wzzfepE-regulated very long O antigen chains
| Autor: | Renato Morona, Gerald L. Murray, Stephen R. Attridge |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Salmonella typhimurium
Salmonella Lipopolysaccharide Iron Immunology lac operon Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology chemistry.chemical_compound Antigen Transcription (biology) medicine Gene Antigens Bacterial Virulence O Antigens Complement System Proteins biology.organism_classification Antibodies Bacterial Enterobacteriaceae Culture Media Infectious Diseases chemistry Bacteria |
| Zdroj: | Microbes and Infection. 7:1296-1304 |
| ISSN: | 1286-4579 |
| DOI: | 10.1016/j.micinf.2005.04.015 |
| Popis: | Salmonella typhimurium possesses two wzz genes conferring long (wzzST) and very long (wzzfepE) lipopolysaccharide O antigen modal chain lengths. While the long O antigen modal length was essential for complement resistance, the very long modal length was found to have a minor role. However, when grown in the presence of serum, S. typhimurium demonstrated a wzzfepE-dependent increase in the density of very long O antigen chains, resulting in a significant increase in serum resistance. Similar phenotypic changes were observed after growth under iron-limiting conditions, though iron limitation is unlikely to be the sole signalling mechanism behind the changes induced in serum. A wzzfepE::lacZ promoter fusion was used to determine that regulation of wzzfepE transcription is unlikely to be the mechanism behind the variation in O antigen length. Since systemic infection occurs in a small but significant percentage of human non-typhoid salmonellosis, the phenomenon identified in this study may be significant during the bacteraemic phase of infection. |
| Databáze: | OpenAIRE |
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