A novel fingerprint method for analyzing the expression of complex multigene families of very low transcript abundance
| Autor: | Klaus Apel, Susanne Bohl |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping DNA Single-Stranded Gene Expression Plant Science Genes Plant law.invention chemistry.chemical_compound Abundance (ecology) law Hordeum murinum Genetics Nucleotide RNA Messenger Polymerase chain reaction Plant Proteins chemistry.chemical_classification Messenger RNA Base Sequence biology RNA Hordeum Cell Biology biology.organism_classification Thionin Genetic Techniques chemistry Multigene Family Polymorphism Restriction Fragment Length DNA Antimicrobial Cationic Peptides |
| Zdroj: | The Plant Journal. 3:887-893 |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/j.1365-313x.1993.00887.x |
| Popis: | Summary A novel RNA fingerprint method has been developed based on the polymerase chain reaction to identify individual mRNA species derived from different members of a complex multigene family of very low trannscript abundance. Using this method, changes in the composition of complex thionin mRNA populations and in the appearance of individual thionin mRNA species in different organs and in response to external stimuli were detected in Hordeum murinum. A single nucleotide exchange within the transcribed DNA may be sufficient to allow the distinction between otherwise identical transcripts. |
| Databáze: | OpenAIRE |
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