Correlação entre a presença de Escherichia coli em lesões de celulite e fígados de frango
| Autor: | R. M. Silva, I. M. M Silva, M. C. Jesus, M. D. B. Fernandes, F. S. Oliveira, J. Evêncio-Neto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
microbial genetics
segurança alimentar e nutricional QH301-705.5 040301 veterinary sciences food and nutritional safety Science sanitary inspection Virulence medicine.disease_cause law.invention Microbiology 0403 veterinary science law Pathogenic Escherichia coli genética microbiana Escherichia coli medicine Animals Biology (General) Pathogen Escherichia coli Infections Poultry Diseases Polymerase chain reaction biology Liver Neoplasms Botany 0402 animal and dairy science Broiler Cellulitis 04 agricultural and veterinary sciences biology.organism_classification medicine.disease 040201 dairy & animal science inspeção sanitária QL1-991 QK1-989 General Agricultural and Biological Sciences Chickens Zoology Brazil Bacteria enterobacteriaceae |
| Zdroj: | Brazilian Journal of Biology, Volume: 81, Issue: 3, Pages: 714-718, Published: 21 SEP 2020 Brazilian Journal of Biology, Vol 81, Iss 3, Pp 714-718 (2020) Brazilian Journal of Biology, Issue: ahead, Published: 21 SEP 2020 Brazilian Journal of Biology v.81 n.3 2021 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE |
| Popis: | Pathogenic strains of Escherichia coli may invade the subcutaneous tissue of poultry and cause cellulitis, whilst the pathogen may also cause lesions in internal organs such as the liver. Current paper co-relates Escherichia coli and virulence genes characteristic of Avian Pathogenic Escherichia coli (APEC) in broilers´ cellulitis and liver lesions. One hundred carcasses were retrieved from the production chain in an avian abattoir in the state of Bahia, Brazil, between August 2013 and January 2014, due to detection of cellulitis lesions. Cellulitis and liver samples were retrieved aseptically to quantify E. coli by Petrifilm™ count fast method (3M Company) (AOAC 998.8). Virulent genes iss and iutA were removed from E. coli isolates by Polymerase Chain Reaction (PCR). Escherichia coli was isolated from 82.0% of broilers removed from the production chain and the bacterium was concomitantly detected in cellulitis and liver lesions in 40.0% of broilers. E. coli counts ranged between 1.00 and 4.73 log CFU/g in liver lesions and between 2.00 and 9.00 log UFC/g in cellulitis lesions. Virulent genes iutA and iss were detected in 97.56% and 89.02% of E. coli isolates, respectively. Genotype analysis demonstrated the concomitant amplification of genes iutA and iss in 60.0% (n=40) of samples of cellulitis and liver lesions in which the simultaneous isolation of E. coli occurred. There was a positive and significant co-relationship (r=0.22; p |
| Databáze: | OpenAIRE |
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