Luteinizing Hormone-β mRNA Levels Are Regulated Primarily by Gonadotropin-Releasing Hormone and Not by Negative Estrogen Feedback on the Pituitary
| Autor: | John W. Funder, Iain J. Clarke, Judith A. Clements, Julie E. Mercer |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
endocrine system
medicine.medical_specialty Somatotropic cell medicine.drug_class Ovariectomy Endocrinology Diabetes and Metabolism Radioimmunoassay Gonadotropin-releasing hormone Biology Gonadotropic cell Feedback Cellular and Molecular Neuroscience Follicle-stimulating hormone Endocrinology Anterior pituitary Internal medicine medicine Animals RNA Messenger Sheep Estradiol Endocrine and Autonomic Systems Nucleic Acid Hybridization Luteinizing Hormone medicine.anatomical_structure Female Corticotropic cell Follicle Stimulating Hormone Gonadotropin Luteinizing hormone Pituitary Hormone-Releasing Hormones hormones hormone substitutes and hormone antagonists |
| Zdroj: | Neuroendocrinology. 47:563-566 |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000124969 |
| Popis: | Long-term effects of gonadotropin-releasing hormone (GnRH) and/or estrogen on pituitary mRNA levels for the beta-subunit of luteinizing hormone (LH-beta) were determined in anterior pituitary glands from ovariectomized (OVX) ewes. The relative roles of these two factors were assessed by studying hypothalamopituitary disconnected (HPD) ewes with appropriate hormonal treatments. Levels of LH-beta mRNA were increased by ovariectomy and substantially reduced by HPD. Treatment of OVX-HPD ewes with pulses of GnRH (250 ng each 2 h) for 1 week restored LH-beta mRNA levels to OVX levels, whereas treatment with estrogen alone did not alter the low levels found in OVX-HPD ewes. Combined GnRH and estrogen treatment for one week produced LH-beta mRNA levels that were similar to those found in OVX-HPD ewes given GnRH alone; plasma LH pulse amplitudes were also similar in these two groups. From these data we conclude that the long-term negative feedback effect of estrogen to reduce LH secretion is due to a primary inhibition of GnRH secretion and is not a pituitary effect of estrogen. Long-term regulation of LH-beta mRNA is thus primarily regulated by GnRH. |
| Databáze: | OpenAIRE |
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