Immunoreaction at 43 kDa with Anti-ubiquitin Antibody in Breast Neoplasms
| Autor: | Takashi Fukutomi, Hiroaki Kono, Takuya Osada, Minoru Suzuki, Seiji Sato, Akihiro Iwamatsu, Yoshihiro Matsuno, Hitoshi Tsuda, Chikao Torikata, Setsuo Hirohashi, Keiichi Iwaya, Hideki Nishibori |
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| Rok vydání: | 1997 |
| Předmět: |
Cytoplasm
Cancer Research Pathology medicine.medical_specialty Molecular Sequence Data Breast Neoplasms medicine.disease_cause Peptide Mapping Epithelium Article Amino acid sequence Ubiquitin medicine Humans Electrophoresis Gel Two-Dimensional Neoplasm Invasiveness Breast Ubiquitins Peptide sequence Actin Neoplasm Staging biology Breast neoplasm Carcinoma Carcinoma Ductal Breast Protein turnover Immunohistochemistry Molecular biology Peptide Fragments Protein ubiquitination Molecular Weight Carcinoma Lobular Oncology Cancer cell Two‐dimensional electrophoresis biology.protein Female Antibody Carcinogenesis |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| Popis: | Protein ubiquitination has been implicated in ATP‐dependent protein turnover and normal cell proliferation. To investigate whether the ubiqnitin‐mediated system is functionally involved in the cancerous state, we examined changes in protein ubiquitination in 52 surgically resected primary breast tumors. Imnmnohistochcmically, ubiquitin (Ub) was identified in the cytoplasm of cancer cells, which were stained more strongly than adjacent normal ductal epithelium. Corresponding immunoblot analysis of normal and neoplastic regions of human breast showed that the immunoreaction for Ub at about 43 kDa was increased in all of the tumors (100%), regardless of the clinical stage or histologic grade. This protein, which gave a single spot on two‐dimensional gel electrophoresis, had partial amino acid sequences which were identical to those of actin family members. Our results suggest that ubiquitination of this 43‐kDa protein may be involved in the carcinogenesis or biological characteristics of human breast neoplasms. |
| Databáze: | OpenAIRE |
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