The armadillo protein p0071 controls KIF3 motor transport
| Autor: | René Keil, Paul Walther, Thomas Seufferlein, Susanne Bobrovich, Marc Porzner, Alexander Becher, Mechthild Hatzfeld, Tim Eiseler |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein subunit Vesicle Secretory Vesicles Signal transducing adaptor protein Kinesins Cell Biology Biology Transport protein Cell biology Vesicular transport protein 03 medical and health sciences Cytoskeletal Proteins Protein Transport 030104 developmental biology HEK293 Cells Microtubule Kinesin Humans Secretion Plakophilins Adaptor Proteins Signal Transducing HeLa Cells |
| Zdroj: | Journal of cell science. 130(19) |
| ISSN: | 1477-9137 |
| Popis: | We here report a novel function of the armadillo protein p0071 (also known as PKP4) during transport mediated by the KIF3 transport complex. Secretion of chromogranin A and matrix metallopeptidase 9 from pancreatic neuroendocrine tumor cells or pancreatic cancer cells, respectively, was substantially reduced following knockdown of p0071. Vesicle tracking indicated that there was impaired directional persistence of vesicle movement upon p0071 depletion. This suggests a disturbed balance between plus- and minus-end directed microtubule transport in cells lacking p0071. p0071 directly interacts with the KIF3 motor subunit KIF3B. Our data indicate that p0071 also interacts with the kinesin cargo adaptor protein KAP3 (also known as KIFAP3) acting as a stabilizing linker between KIF3B and its KAP3 cargo-binding entity. Thus, p0071 is required for directional vesicle movement and secretion of different KIF3-transported carriers, thereby regulating the transport of intracellular membrane vesicles along microtubules. |
| Databáze: | OpenAIRE |
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