An HRP-based amperometric biosensor fabricated by thermal inkjet printing
| Autor: | A. Filippini, Barbara Ballarin, Alessandro Fraleoni-Morgera, Leonardo Setti, M. Di Biase, I. Mencarelli |
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| Přispěvatelé: | L. Setti, A. Fraleoni-Morgera, I. Mencarelli, A. Filippini, B. Ballarin, M. Di Biase, Leonardo, Setti, FRALEONI MORGERA, Alessandro, Ivan, Mencarelli, Alessandro, Filippini, Barbara, Ballarin, Manuela, Di Biase |
| Rok vydání: | 2007 |
| Předmět: |
Materials science
Electronic ink INKJET PRINTING Nanotechnology Bioelectronic Horseradish peroxidase PEDOT:PSS Biopolytronic Materials Chemistry Thermal inkjet printing Semipermeable membrane Electrical and Electronic Engineering Instrumentation Electrical conductor chemistry.chemical_classification Bioelectronics biology Metals and Alloys Polymer Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials BIOPOLYTRONICS Biosensors chemistry Electrode biology.protein BIOSENSORS HORSERADISH PEROXIDASE Biological ink Biopolytronics Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 126:252-257 |
| ISSN: | 0925-4005 |
| Popis: | Direct inkjet printing of a complete and working amperometric biosensor for the detection of hydrogen peroxide, based on horseradish peroxidase (HRP), has been demonstrated. The device has been realized with a commercial printer. A thin layer of PEDOT:PSS, which was in turn covered with HRP, was inkjet printed on top of an ITO-coated glass slide. The active components of the device retained their properties after the thermal inkjet printing. The whole device has been encapsulated by means of a selectively permeable cellulose acetate membrane. The successful electron transfer between the PEDOT:PSS covered electrode and the enzyme has been demonstrated, and the biosensor evidenced very good sensitivity, in line with the best devices realized with other techniques, and a remarkable operational stability. This result paves the way for an extensive application of “biopolytronics”, i.e. the utilization of conductive/semiconductive polymers and biologically active molecules to design bioelectronic devices using a common PC, and exploiting normal commercial printers to print them out. |
| Databáze: | OpenAIRE |
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